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C. Adjusting the aperture of the diaphragm.
The diaphragm (8) should not be used to regulate the light intensity, its function is to obtain a
high resolution of the specimen and to provide contrast in the image. Smaller apertures will
deliver higher contrast to image, although closing the aperture too much will reduce
resolution. The best way to obtain the correct aperture of the diaphragm is to experiment.
Suggested apertures for each objective are as follows:
D. Changing magnification.
1. Position the objective 10X (4) in the optical path.
2. This microscope has already been parfocalised, although it is possible that small differences
exist between the objectives. It may then be necessary to adjust the focus slightly with the
fine focus knob (15).
3. When changing to the 40X and 100X objectives (optional), it must be done with great care,
ensuring that the objectives do not cause damage to the front lens.
4. In order to obtain maximum resolution of the 100X objective (optional), it is necessary to
apply immersion oil between the cover slip of the slide and the front lens of the objective.
NB. Immersion oil must ONLY be used with the 100X objective, as it is the only one
specially prepared for it. If any other objective comes into contact with the oil, it must
be cleaned immediately.
Maintenance
WARNING: FOR YOUR OWN SAFETY SWITCH OFF AND DISCONNECT THE MICROSCOPE FROM
ANY ELECTRICAL SOURCE, BEFORE ATTEMPTING ANY MAINTENANCE PROCEDURE TO AVOID
THE RISK OF ELECTROCUTION.
IF THE MICROSCOPE REQUIRES ANY MAINTENANCE OR REPAIR NOT APPEARING IN THIS
MANUAL, CONSULT YOUR DISTRIBUTOR.
A. Optical maintenance
Do not attempt to disassemble any optical component.
Prior to cleaning any of the lens surfaces, remove dust particles using a fine brush, specifically for
cleaning lenses. Alternatively, use low pressure compressed air, available in shops selling
photography equipment.
OBJECTIVE
4X
10X
40X
100X (optional)
a. Only a very small amount of immersion oil is needed, a drop should be enough.
b. If air bubbles appear they can be removed by gently rotating the nosepiece (3)
back and forth.
c. After viewing, all parts that have come into contact with the oil must be cleaned
using a soft cotton cloth, lightly dampened with Xylene. If the 100X objective is not
cleaned, the oil will dry, and it will not be possible to see through it; permanent
damage could also occur.
APERTURE OF IRIS
From fully closed to 1/8 open.
From 1/8 to 1/4
From 1/4 to 1/2
From 1/2 to 3/4
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