English; Setting The Volume; Method Of Pipetting; Recommendations - HTL LABMATE pro Manual Del Usario

2 - SETTING THE VOLUME

The volume of the pipette is set by the knob in the pipet-
ting pushbutton (Fig. 1A2) or by the black adjustment
knob (Fig. 1B). The volume shown by the counter is rep-
resented by three digits, which should be read from top
to bottom. Typical meter readings are shown in the fol-
lowing table:
Counter
Model
readings
0
LMP8-10
3
LMP12-10
5
0
LMP8-50
6
LMP12-50
5
0
LMP8-200
8
LMP12-200
5
2
LMP8-300
5
LMP12-300
0
To attain the maximum accuracy, set volume must be
approached from a higher value by diminishing counter
readings.
• If the desired volume is lower than set volume shown
by the counter, the operator should turn the pipetting
pushbutton (Fig. 1A2) or the black adjustment knob
(Fig. 1B) to the direction diminishing counter readings
to the required volume. Before achieving the required
volume slowly rotate the knob and observe carefully
diminishing reading to avoid accidentally passing the
setting value.
• If the desired volume is higher than set volume shown by
the counter, the operator should turn the pipetting push-
button (Fig. 1A2) or the black adjustment knob (Fig. 1B)
increasing the value until the lower figure wheel comes
1/3 of a turn beyond the required setting and then slowly
backward until the setting reaches the desired volume.
Make sure not to pass the setting value.
If the knob is accidentally turned too far, the process
must be repeated. The desired volume must always be
set from the higher value in the order of decreasing value.
3
Set Basic
volume degree
3.5 μl 0.02 μl
6.5 μl 0.10 μl
85.0 μl 0.20 μl
250.0 μl 1.0 μl

3 - METHOD OF PIPETTING

Fit the tips onto cones of the multichannel mo dule. In
order to put tips on the multichannel shafts hold the
pipette vertically and press it against the tips in the rack
box, until the shafts retreat about 1.5 mm into the multi-
channel module. The suspension system of the shafts
ensures even and tight sealing of tips. The rolling move-
ment does not have to be performed to seal the tips
tightly. The liquid aspirated into the tips should not flow
out by gravity from properly fixed tips. While holding the
pipette in a vertical position, tips should be immersed in
the liquid to a depth of 2-4 mm, and flushed once by
drawing a dose of liquid and dispensing it out with slow
and steady movement. Next, still holding the pipette ver-
tically, the ope ra tor should press the pipetting button
until the first resistance point is felt, and immerse the tips
in the liquid to the depth of 2-4 mm, (Fig. 3B). Rele asing
the pipetting button with a slow and uni form movement
during 2-3 seconds, the liquid should be drawn into the
tips, (Fig. 3B) which should then be lifted above the liq-
uid's surface. The pipette should next be positioned at an
angle of 10-45 degrees in relation to inner walls of desti-
nation vessels and tips should be emptied by pressing
the pipetting button slowly until the first resistance point is
felt, (Fig. 3C). After a second, the pipetting button should
be pressed to the second resistance point in order to
expel remaining liquid, (Fig. 3D). Then tips should be lift-
ed out from the vessels while maintaining contact between
the ends of the tips and the inner walls of the vessel until
the pipetting button has been rela sed, (Fig. 3E). Finally,
pressing the ejector button, detach the tips, (Fig. 3F).

4 - RECOMMENDATIONS

To achieve maximum safety, precision and re lia bility, the
following principles should be ob served:
• do not draw liquid without tips attached,
• do not lay down the pipette with tips filled,
• do not draw volumes of liquid exceeding the pipette's
range,
• check if the tips are fitted properly,
•during operation, the pipette must be held vertically,
tips should be immersed in liquid to the depth of 2 to 4
milimeters and the pipetting button should be depres -
sed and rele ased slowly and evenly,

ENGLISH

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