Centering The Field Diaphragm; Using The Microscope; Switching Fluorescence Filter Cubes - Optika Italy IM Serie Manual De Instrucciones

14.2 Centering the field diaphragm
Only IM-3F / IM-3FL4
1. Place the specimen on the stage, insert 10x objective
into the light path and focus.
2. Rotate the field diaphragm lever ①, to fully close the
diaphragm. (Fig. 63)
3. Rotate the two centering screws ② to bring the bright
spot in the center of the field of view.
4. Gradually open the diaphragm. The field diaphragm is
centered when the diaphragm image is symmetrical to
the field of view.
5. In normal use, open the diaphragm until it circoscribes
the field of view.
Effects of the field diaphragm
Field diaphragm adjusts the illuminated area to obtain a
high contrast image.
Set the diaphragm according to the objective in use
until it circoscribes the field of view, in order to eliminate
unnecessary light to eyepieces.(Fig. 64)

14.3 Using the microscope

• IM-3F / IM-3FL4
Turn on the power supply for the mercury bulb and wait 5
minutes for the arc to stabilize. (Fig. 55)
• IM-3LD / IM-3LD4 / IM-3LD4D
Move the main switch in the position "I" to turn on the
microscope, then rotate the adjustment knob ③ (Fig. 65) to
adjust the fluorescence light intensity.
14.3.1 Switching fluorescence filter cubes
• IM-3F
Move the filter levers (placed on the right or on the left
sides of the microscope) ④ to insert the desired filter block
(B or G). (Fig. 66)
③
④
Page 39
② ①
F ig. 63 F ig. 63
F ig. 64 F ig. 64
F ig. 65 F ig. 65
F ig. 66 F ig. 66