StarLab ErgoOne Manual De Usuario página 14

Implementation
a) Adjust the pipette to the required nominal volume.
b) C arry out ten pipettings with subsequent weighing and record the
individual measurements.
c) Calculate the mean value:
d) Calculate the corresponding volume:
The calculated volume corresponds to the actual setting of your
pipette (the density of water at 20°C = 0.9982 g/ml).
e) I f realignment is necessary, open the volume adjustment catch
by pulling up the volume control button
Remove the cover of the alignment unit
using a pointed object or pen (with the nib retracted), and (whilst
holding the button down) turn the volume control button
change the piston stroke of the pipette (the volume display re-
mains unchanged). Turn the volume control button
increase the volume, counter clockwise to reduce the volume (see
table 2 for details). Once alignment is complete, push the volume
control button
alignment unit
A full rotation of the volume control button
following volume change:
Volume change
ErgoOne® Single-Channel
0.1 – 2.5µl
0.5 – 10µl
2 – 20µl (micro cone)
2 – 20µl (standard cone)
10 – 100µl
20 – 200µl
100 – 1000µl
500 – 5000µl
ErgoOne® Multi-Channel
0.5 – 10µl
10 – 100µl
30 – 300µl
14 ErgoOne
down again until you hear a click. Replace the
1
cover, and the pipette is ready for use again.
3
Volume change approx.
0.13µl
0.7µl
1.4µl
1.4µl
7µl
14µl
70µl
340µl
Volume change approx.
0.7µl
7µl
14µl
User Manual
®
∑ xi
Mean value x =
n
xi = weighing results
n = number of individual weighings
x (mg)
Volume (µl) =
Density of liquid
x = calculated mean value
until you hear a click.
1
. Slide the button down,
3
clockwise to
1
corresponds to the
1
Note:
Multi-channel pipettes are
only aligned on one channel.
Attach a single tip to any
channel and proceed as
described above.
to
1