Preparation For Operation; Pipetting Recommendations - HTL DISCOVERY PRO Manual Del Usuario

A B
Fig. 2
The range of aspirated volume is displayed on the
counter cap (fig. 1G). The volume is displayed by the
counter and consists of four digits, which should be read
from top to bottom. The digits on the lower drum consti-
tute minimum graduation for the particular model. Value
of the minimum graduation (increment) is presented in
Table 1.
Volume can be changed after choosing the upper posi-
tion of the knob (fig. 2A and 2B).
After setting the desired volume, the knob (fig. 1B) should
be locked by pushing into the lower position (fig. 2C).
Examples of counter indications:
0.5-10 μl 5-50 μl
0.5 μl 10 μl 5 μl
Counter indications above the horizontal indicators shown
volume in [μl].
To achieve maximum accuracy, the required volume
should be set from higher volume by reducing indica-
tions of the counter.
Example of correct volume setting:
• If the required volume is lower than
the volume set on the counter it is
necessary to reduce indications of
the counter to the required value.
Before achieving the required value it
is necessary to reduce the speed
5
C
50-300 μl
50 μl 50 μl 300 μl
Fig. 3
with which you rotate the adjustment knob (fig. 1B), so
as not to exceed the value (fig. 3).
• If the required volume is higher than the volume set on
the counter it is necessary to increase indications of the
counter to the value which exceeds the required volume
by rotating the bottom drum by about 1/3 of its circum-
ference. Then, by slowly rotating the adjustment knob
(fig. 1B), reduce the setting to the required value (fig. 3).
After setting the required volume, the knob (fig. 1B) should
be moved downward to lock the set volume (fig. 2C).
CAUTION: The calibration switch (fig. 1E) which is above
the counter should remain in the lower position during
operation. Otherwise, each change of capacity will result in
decalibration of the pipette.

2.2. PREPARATION FOR OPERATION

Place the tips on the shafts of the pipette. Holding the
pipette vertically, press it against the tips in the rack box,
until the shafts retreat about 1.5 mm into the multichannel
module. The suspension system of the shafts ensures
even and tight sealing of the tips.
The twisting motion does not have to be performed.
See Table 1 for the appropriate tips designed for the given
pipette model.
CAUTION:
- It is recommended to use the tips recommended by the
manufacturer, as only those tips will ensure accurate
and repeatable liquid measurements.
- Never aspirate liquid into a pipette without tips attached.

3. PIPETTING RECOMMENDATIONS

Observing the following recommendations will ensure
accuracy and repeatability of liquid sampling.
• While operating DISCOVERY PRO pipette the volume
setting should be locked - the adjustment knob in its
lower position (fig. 2C).
• Operate the pipette pushbutton slowly and smoothly
when liquid aspiring and dispensing.
• Pipette is equipped with a switch of counter's mode of
operation – calibration switch (fig. 1E). This switch
should be shifted only for the time of calibrating the
pipette (upper position marked with the "CAL" symbol).
ENGLISH
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