Aspirating And Dispensing Liquids; Aspirating Liquids; Dispensing Liquids; Pre-Rinsing - HTL DISCOVERY PRO Manual Del Usuario

• The depth of tips immersion in the aspirated liquid
should be around 2-4 mm.
• While operating, the pipette should be held in a vertical
position.
• New tips should be pre-rinsed.
• The tips should be replaced with new ones if:
- a different type of liquid is handled;
- a different liquid volume is dispensed;
- visible droplets of liquid remain in them.
• Pipetted liquid should not enter the pipette shafts. To
ensure this:
- press and release the pipetting pushbutton slowly
and smoothly;
- do not put the pipette away if there is any liquid in the
tips;
- do not invert the pipette;
- do not set and aspirate volumes exceeding nominal
values.
• Before aspirating liquid of temperature that differs from
ambient temperature, it is recommended to rinse the
tips several times with the aspirated liquid.

4. ASPIRATING AND DISPENSING LIQUIDS

4.1 ASPIRATING LIQUIDS

A B C
Fig. 4
• The pipetting pushbutton (fig. 1A) should be pressed to
the first stop (fig. 4A),
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D E F
• While holding the pipette in a vertical position, tips
should be immersed into the aspirated liquid to the
depth of 2 to 4 mm, and flushed once by drawing
a dose of liquid and dispensing it out with slow and
steady movement. The tips may aspirate air if immer sed
to a depth lower than recommended or if the pipetting
pushbutton is released quickly,
• To aspirate liquid release the pipetting pushbutton
smoothly (fig. 4B),
• Wait for about 1 second before withdrawing the tips
from the aspirated sample, fig. 4.
WARNING! Do not touch the used tips.

4.2 DISPENSING LIQUIDS

• While holding the pipette slightly inclined from 10 to 40°
off the vertical, place the tips orifice against the inside
wall of the receiving vessel.
• Then, press the pipetting pushbutton smoothly to the
first stop, dispensing the liquid (fig. 4C).
• After about one second press the pipetting pushbutton
to the final stop, blowing out the remaining liquid from
the tips (fig. 4D).
• Holding the pushbutton depressed remove the pipette,
drawing it against the vessel inner wall.
• Release the pipetting pushbutton to its starting position
(fig. 4E) and eject the tips by pressing the tip ejector
button (fig. 4F).
CAUTION: Replace the tips with a new one whenever a
diffe rent liquid is to be pipetted.

4.3 PRE-RINSING

When pipetting liquids of viscosity higher or surface
tension lower than those of water (e.g. serum or organic
solvents) a film of liquid is formed on the inside wall
of the pipetting tips. The film can create an error. Since
the film remains relatively constant in successive pipetting
operations with the same tips, this error can be avoided
by forming the film before the first pipetting. This is done
by performing a full pipetting cycle of a liquid into the
same vessel. After such a procedure, the film of liquid is
already formed in the tips and ensures better accuracy
and repeatability of successive pipetting operations.
ENGLISH
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