15. Troubleshooting
Review the information in the table below to solve operating problems.
PROBLEM
I. Optical Section:
LED operates, but field of view remains
dark.
Field of view is obscured or not evenly
illuminated
Dirt or dust is visible in the field of view. Dirt/dust on the specimen
Image looks double
Visibility is poor.
•
Image is poor.
•
Contrast is poor.
•
Details are indistinct.
One side of the image is unfocused
II. Mechanical Section:
Coarse focus knob is hard to turn
Focus is unstable
III. Electrical Section:
LED doesn't turn on.
Brightness is not enough
Light blinks
IV. Microphotography:
Image edge is unfocused
CAUSE
Microscope power supply is unplugged Connect
Brightness setting is too low
Camera power supply is unplugged
Monitor power supply is unplugged
Camera is turned off
Camera exposure parameters are not
correctly set
Switching objective the microscope is
not parfocal
Revolving nosepiece is not correctly
engaged.
Aperture iris diaphragm is stopped
down too far.
The condenser is not well centered or
it is in a wrong height
Revolving nosepiece is in an incorrect
position
Aperture iris diaphragm is too closed
or too open.
Dust or dirt on lenses (condenser, ob-
jectives and slide)
For transmitted light observation, the
coverglass thickness must not exceed
0.17mm
Focus is not even
Revolving nosepiece is in an incorrect
position
Slide is mounted not in a flat position
(tilted)
Poor quality of the glass slide
Tension adjustment ring is too tight
Tension adjustment ring is too loose
Power supply not connected
Brightness setting is too low
Camera exposure time set incorrectly
Power supply not well connected
To a certain extent it is due to achro-
matic objectives features
Page 33
SOLUTION
Adjust brightness
Connect
Connect
Turn on camera
Set camera with a proper exposure
Perform parfocality adjustment again
Make sure that the revolving nose-
piece clicks properly into place.
Clean the specimen
Open aperture iris diaphragm.
Set the condenser according to Koe-
hler settings.
Move the nosepiece to a click stop
Adjust aperture iris diaphragm.
Clean thoroughly
Use a coverglass with thickness
0.17mm
Slide holder is not flat. Move the spec-
imen to a flat position
Move the nosepiece to a click stop
Place the specimen in a flat position
on the stage
Use a glass slide with higher quality
Loosen tension adjustment ring
Tighten tension adjustment ring
Check for proper connection
Adjust brightness
Adjust exposure time
Check for proper connection
To minimize the problem, set the aper-
ture diaphragm in a proper position