A
PPLICATIONS
The SERO 12 has been designed to facilitate blood testing procedures involving centrifugation, incubation, and cell
washing with minimal tube handling. Many procedures can be performed entirely without removing tubes from the
centrifuge rotor, thus reducing the possibility of errors in transferring tubes. The following notes on use of the SERO
12 are intended to set practical guidelines for the technologist. When possible, refer to the manufacturer's I.F.U.
A
T
GGLUTINATION
ESTS
Whenever possible, it is important that the anti-serum manufacturer's instructions be followed on recommended
time periods for centrifuging cells with their sera in the SERO 12.
NOTE: It is recommended that the optimum centrifugation period be determined by controlled reactions using
known cells. This method is the easiest way to determine time necessary to produce the desired tightness of
agglutination.
I
NCUBATION
Many blood testing procedures require incubation at 37°C before and after centrifugation. The SERO 12 rotor has
been designed to allow the incubation of these reactions without removing the tubes. You may lower the entire
rotor into a water bath for the test being performed. When the rotor is removed, water will drain through the
perforations in its base. Allow the water to drain completely prior to transporting tubes.
C
W
ELL
ASHING
The SERO 12 rotor may be conveniently used for many tests that require single or multiple blood cell washings.
Because the tubes are maintained at an angle of 45° during centrifugation, the centrifuge is highly efficient in
washing cells. The cells are deposited quickly after sliding down the angled tubes.
After the button is formed, the supernatant solution can be readily poured off all tubes simultaneously. This is done
by removing the rotor from the drive spindle immediately after it stops spinning, placing the retainer ring around
the tubes, and inverting the rotor.
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