Aspiration And Dispensing Liquids - HTL CLINIPET+ Manual Del Usario

Accu -
Pipette Cat. Volume
racy
model number [μl]
A [%]
CP 2 2202 2 ±3.0
CP 5 2204 5 ±2.0
CP 7 2206 7 ±1.5
CP 10 2208 10 ±1.2
CP 15 2210 15 ±1.0
CP 20 2212 20 ±0.9
CP 25 2214 25 ±0.9
CP 30 2216 30 ±0.9
CP 40 2218 40 ±0.9
CP 44.7 2220 44.7 ±0.9
CP 50 2222 50 ±0.9
CP 60 2224 60 ±0.9
CP 70 2226 70 ±0.9
CP 75 2228 75 ±0.9
CP 80 2230 80 ±0.9
CP 90 2232 90 ±0.8
CP 100 2234 100 ±0.8
CP 120 2236 120 ±0.8
CP 150 2238 150 ±0.7
CP 200 2240 200 ±0.6
CP 200A 2242 200 ±0.6
CP 220 2244 220 ±0.6
CP 250 2246 250 ±0.6
CP 300 2248 300 ±0.6
CP 400 2250 400 ±0.6
CP 450 2252 450 ±0.6
CP 500 2254 500 ±0.6
CP 600 2256 600 ±0.6
CP 700 2258 700 ±0.6
CP 750 2260 750 ±0.6
CP 800 2262 800 ±0.6
CP 900 2264 900 ±0.6
CP 1000 2266 1000 ±0.6
The accuracy and precision figures are ob tai ned
using HTL tips, using a gravimetric method, per-
forming at least 10 measurements of distilled
water at the temperature of 20±1°C, according
to EN ISO 8655 standard.
The pipette design enables the user to perform
the recalibration process according to the rules
presented in section 5.
Volume change V is the change of the distilled
water volume as pirated by the pipette caused
by a single com plete turn of the calibration key
- 24 increments.
3
Volume
Precision change Tip Shaft
P [%] V volume colour
[μl]
±1.5
±1.0
0.7
±0.7
±0.5
±0.5
1.3
±0.4
1.7
±0.4
±0.3
±0.3
3.5
±0.3
200 μl yellow
±0.3
±0.3
±0.3
±0.3
7.0
±0.3
±0.3
±0.3
±0.3
13.0
±0.3
±0.2
±0.2
±0.2
±0.2
31.0
±0.2
±0.2
±0.2
1000 μl blue
±0.2
±0.2
±0.2
±0.2
60.0
±0.2
±0.2
± 0.2

2 - ASPIRATION AND DISPENSING LIQUIDS

Attaching the tip
• Select an appropriate tip (see Section 3).
• Press the ejector pushbutton with your thumb
from the top, (Fig. 2A).
• Pressing the ejector pushbutton, push the pi -
pette cone into tip opening, (Fig. 2B).
NOTE! Never aspirate liquid into a pipette
without a tip attached.
Pre-rinsing the tip
When a tip will be used for the first time, espe-
cially when pipetting liquids of viscosity or sur-
face tension different from water (e.g. serum, or -
ga nic solvent), inner surface of the tip is wetted
with the liquid remaining inside. Wetting inner
surfaces causes errors, as the volume of the
first dispensed sample will be smaller than that
of successive samples. To avoid those errors,
the tip should be pre-rinsed with the pipetted
liquid before the first sample is dispensed. This
is accomplished by aspirating a sample of the
required volume and dispensing it back. When
the tip is pre-rinsed, the next pipetted volume
will be free of any wetting error.
Aspiration of liquid
• Press the pushbutton slowly and smoothly to
the first stop, (Fig. 3A).
• Holding the pipette vertically, immerse the tip
into the sampled liquid. The tip immersion
depth depends on the tip used, and the sam-
pled volume.
Tip [μl]
200
1000
• Release the pipetting pushbutton slowly and
uniformly, aspirating liquid into the tip, (Fig. 3C).
• Wait one second and withdraw the tip from
the liquid.
Liquid Dispensing
• Hold the pipette slightly inclined (10÷40° off
the vertical), and place the tip orifice against
the inside wall of the receiving vessel.
ENGLISH
Immersion depth [mm]
2÷3
2÷4
4