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SPECIFICATIONS
Shipping Solvent
•
Kinetex C18, EVO C18, XB-C18, C8, Biphenyl, Phenyl-Hexyl, F5 and
PFP columns are shipped in Acetonitrile / Water (≥ 50:50 v/v; exact
composition dependent on column dimensions)
•
Kinetex HILIC is shipped in Acetonitrile / Water 90:10 (v/v)
Test Certificate
Each Kinetex C18, EVO C18, XB-C18, C8, Biphenyl, Phenyl-Hexyl, F5
and PFP column is individually tested before shipment. A test certificate
showing the separation parameters for the reversed phase test mixture
containing uracil, acetophenone, toluene, and naphthalene can be found
online at www.phenomenex.com/mysupport.
Each Kinetex HILIC column is individually tested before shipment. A test
certificate showing the separation parameters for the HILIC test mixture
containing toluene, uracil, and cytosine can be found online at
www.phenomenex.com/mysupport.
If the performance of your Kinetex column is not similar to the test
certificate, please review the system optimization tips in this guide or
contact Phenomenex.
OPERATING PARAMETERS
Column Installation
The arrows on the column tag indicate the flow direction. Do not operate
or back-flush a Kinetex 1.3 µm column in the opposite direction to the one
indicated by the arrow on the column tag. Phenomenex recommends the
use of HPLC / UHPLC Sure-Lok
installation of Kinetex columns on HPLC / UHPLC systems. The convenient
one-piece design (AQ0-8503) is pressure rated to 12,000 psi (827 bar),
while the 3-piece unit (AQ0-8504 with AQ0-8505), which contains a
PEEK nut, a ferrule, and stainless steel gripping ring, will provide leak-free
connections up to 19,000 psi (1,310 bar). A handy fitting tightening
tool (AQ0-8530) is available to facilitate achievement of a leak-free
connection.
Mobile Phase Restrictions
•
Kinetex EVO C18 columns are stable from pH 1 - 12 and can be used
with typical reversed phase mobile phase (aqueous methanol, aqueous
acetonitrile or appropriate aqueous buffer / methanol or aqueous
buffer / acetonitrile mixtures)
•
Kinetex C18, XB-C18, C8, Biphenyl and Phenyl-Hexyl columns are
stable from pH 1.5 to 10* and can be used with typical reversed phase
mobile phase (aqueous methanol, aqueous acetonitrile or appropriate
aqueous buffer / methanol or aqueous buffer / acetonitrile mixtures)
•
Kinetex F5 and PFP columns are stable from pH 1.5 to 8.5 and can be
used with typical reversed phase mobile phase (aqueous methanol,
aqueous acetonitrile or appropriate aqueous buffer / methanol or
aqueous buffer / acetonitrile mixtures)
•
Kinetex HILIC columns are stable from pH 2.0-7.5 and can be used
with typical HILIC mobile phase (acetonitrile / aqueous buffer mixtures)
* For isocratic conditions only.
Use only high purity reagents and high quality chromatography grade
solvents to prepare mobile phase. Trace impurities can dramatically
degrade column lifetime. Degas and filter all mobile phase prior to use.
Ensure sample (and matrix) are completely soluble / miscible with mobile
phase. Immiscible solvents or buffer salt precipitation can permanently
damage the column.
High Pressure PEEK male nut fittings for
™
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