Ordering Information - Phenomenex KITENEX Guía Para El Cuidado Y Optimización

System Optimization
Kinetex 2.6 µm core-shell columns operate comfortably within
®
the pressure limits of conventional HPLC instruments and rival the
performance of sub-2 µm fully porous particles achieved on UHPLC
instruments. However, to maximize the benefits from your Kinetex
core-shell column do the following:
•
Minimize sample dispersion before the column
1. Gradient elution will minimize dispersion
2. In isocratic elution, use an injection solvent that's weaker than
your mobile phase
•
Optimize detector settings by adjusting the scan rate and/or the time
constant to the fastest possible setting such that signal-to-noise
(s/n) is not adversely affected.
•
Minimize the extra-column volume between the injector and the
column, and between the column and the detector
1. Minimize the length of all connection tubing
2. Use 0.12 mm ID (0.005 in.) tubing whenever possible
a) 0.17 mm ID (0.007 in.) tubing is acceptable
b) Avoid, if possible, using 0.25 mm ID (0.010 in.) tubing
3. Use extremely low dead-volume fittings
4. Ensure all connecting tubing is seated properly at every
connection
•
Use a micro volume flow-cell
1. Standard flow cells on conventional LC systems can be >10 µL
2. For best results, replace standard flow cells with <3 µL flow cells
(<2 µL when using 2.1 mm ID columns)
NOTE: To convert existing HPLC and UHPLC methods for use on Kinetex
columns we have created a conversion calculator, available on the
Phenomenex website:
Extending Column Lifetime
Phenomenex recommends the use of the SecurityGuard
guard cartridge system to extend the lifetime of your Kinetex column,
especially with samples extracted from complex matrixes. Ideally,
samples must be completely dissolved in the mobile phase or filtered
through a syringe filter of approximately 0.20 µm porosity.
SecurityGuard ULTRA Cartridge Holder

Ordering Information

Part No. Description
AJ0-9000 SecurityGuard ULTRA Cartridge Holder
Cartridge Holder
SecurityGuard ULTRA Cartridges
Ordering Information
Cartridges (3pk)
Material
EVO C18
C18
C8
PFP
F5
Biphenyl
HILIC
Phenyl
Terms and Conditions
Subject to Phenomenex Standard Terms and Conditions which may be viewed at
www.Phenomenex.com/TermsAndConditions
Trademarks
Kinetex is a registered trademark, SecurityGuard is a trademark of Phenomenex.
Kinetex EVO is patented by Phenomenex. U.S. Patent Nos. 7,563,367 and
8,658,038 and foreign counterparts
Phenomenex products are available worldwide.
www.phenomenex.com/mysupport
© 2015 Phenomenex, Inc. All rights reserved.
www.phenomenex.com/optimize
2.1
AJ0-9298
AJ0-8782
AJ0-8784
AJ0-8787
AJ0-9322
AJ0-9209
AJ0-8786
AJ0-8788
Column ID (mm)
3.0
AJ0-9297
AJ0-8775
AJ0-8777
AJ0-8780
AJ0-9321
AJ0-9208
AJ0-8779
AJ0-8781
ULTRA
™
Unit
ea
4.6
AJ0-9296
AJ0-8768
AJ0-8770
AJ0-8773
AJ0-9320
AJ0-9207
AJ0-8772
AJ0-8774