Using The Pampa Evolution96 Software And Biomek Fx Workstation; Preparing A Blank - Beckman Coulter Biomek FX-ADMETox Manual Del Usuario

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Using the PAMPA EVOLUTION96 Software and Biomek FX Workstation

Preparing a Blank

Default file which contains the UV spectra of a Blank may be replaced with new one.
1. Launch PAMPA Evolution-96 Command Software on the computer connected to the
PAMPA Evolution instrument.
2. Go to menu File | Save as and save new file as ""YYMMDD_Blank" (for example:
040701_Blank) where the first 6 characters are the year-month-day numbers.
3. Be careful not to touch the underside of the UV plates, place 4 new 96-well plastic UV
plates stacked on top of each other into position P 11.
4. Put High Profile, 12 Trough Reservoir filled with System Solution into position P4.
5. Click the Start Assay button on the toolbar. The Start Assay dialog displays. Make sure
that all check-boxes are not selected.
6. Click the Start button.
7. A dialog box displays Transfer the System Solution to UV plate.
When the instrument finishes this step, Spectrophotometer will start reading UV spectra of
the blank plate. Wait till Spectrophotometer finishes reading.
To inspect the spectrophotometric data, click View 96 Spectra button.
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2. 96 spectra view displays.
3. Inspect UV spectra of the blank in order to exclude bacterial contamination.
4. In 96 spectra view go to menu File | Export Plate Spectra
5. The Save Spectra dialog box displays.
6. Select Blank Plate from Plate to be Exported drop-down list.
7. Click the Save button.
8. A special spectra file will be saved in the same folder where data file is under the name
Data file name_Blank Plate. If appropriate care is taken of the System Solution, one
Blank may be reused for many experiments.
9. Abort the assay and close the method.
10. Start a new PAMPA method and go to the main menu Instrument | Settings.
11. The internal UV blank spectrum must be loaded into the file.
12. Browse for the file Data file name_Blank Plate.spc and click the Open button.
13. Select Use pre-measured blank.
14. Select Tell me about low -UV wells...
15. Select Auto-refine. The results of the experiment will have the results automatically
calculated.
16. Click the Ok button when done.
Biomek FX/PAMPA Evolution96 Quick Start and Trouble Shooting Guides
PN A29703AB
Using the PAMPA EVOLUTION96 Software and Biomek FX Workstation
Quick Start Guide
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Quick Start Guide
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