Troubleshooting - bioSan Assist Serie Manual De Instrucciones

The single channel pipettes
Range
Preset
of the pipette
Model volume
volumes
[µl]
[µl]
AP2 0.1 - 2 0.2
AP10 0.5 - 10 0.5
AP20 2 - 20 2
AP50 5 - 50 5
AP100 10 - 100 10
AP200 20 - 200 20
AP250 50 - 250 50
AP1000 100 - 1000 100
AP5000 500 - 5,000 500
AP10000 1,000 - 10,000 1,000
The multichannel pipettes
Range
Preset
of the pipette
Model volume
volumes
[µl]
[µl]
AP8-10
0.5 - 10 0.5
AP12-10
AP8-50
5 - 50 5
AP12-50
AP8-200
20 - 200 20
AP12-200
AP8-300
50 - 300 50
AP12-300
• Perform 5 aspirations, weigh each one and calculate the
average value of the aspirations
• Perform three aspiration series (each series should include
the aspirations from all channels), weigh each time and
calculate the average value of the aspirations.
• Calculate average aspirated volume in µl multiplying the
average aspiration amount [mg] by the distilled water density
coefficient [µl/mg], which depends on temperature and
pressure according to the following table:
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Volume change
Permissible DV for full turn
volumes of the calibra-
[µl] tion key [µl]
(24 increments)
0.176 - 0.224 0.06
0.48 - 0.52 0.33
1.94 - 2.06 0.63
4.875 - 5.125 2.50
9.84 - 10.16 2.50
19.76 - 20.24 6.30
49.5 - 50.5 6.30
98.4 - 101.6 25.00
494 - 506 125.00
975 - 1,025 250.00
Volume change
Permissible DV for full turn
volumes of the calibra-
[µl] tion key [µl]
(24 increments)
0.45 - 0.55 0.33
4.8 - 5.2 1.67
19.4 - 20.6 6.30
49.2 - 50.8 10.00
Temperature
[°C]
95.0
20 1.0028
21 1.0030
22 1.0032
23 1.0034
24 1.0037
25 1.0039
If the average aspirated volume exceeds the permissible
value, the following should be done:
• Remove the pipetting pushbutton, (Fig. 4A),
Warning: The pipetting pushbutton consists of 2 parts: the
lower volume-setting section (Fig. 1A2) and the upperbutton
(Fig. 1A1). After removal of the pushbutton, both parts will
separate.
• Holding the volume setting knob to protect it against rotation,
insert the calibration key into the cuts of the calibration
screw, (Fig. 4B),
• Turn the key clockwise to reduce the aspirated volume, or
counter-clockwise to increase the volume. One full turn of
the calibration key changes the pipette aspiration volume
by the amount given in the table, (Fig. 4C),
• Take out the key and fix the pipetting pushbutton (Fig.
4D). The pipetting pushbutton should be fixed by placing
the lower section on the arbor first (Fig. 1A2) and then the
button (Fig. 1A1).
Determine the average aspirated volume. The average volume
should be within the permissible range shown in the table.
If the volume exceeds the values stated, the recalibration
procedure should be repeated.
When pipetting liquids with physical properties considerably
different from those of water, follow the rules as described
in section 5.
9 – TROUBLESHOOTING
If you notice an improper pipette operation identify the cause
and eliminate the fault. To do this, follow the instruction ac-
cordingly. Replacement of parts should be required only
occasionally, and should not occur under normal pipette use.
ENGLISH
Pressure [kPa]
101.3 105.0
1.0029 1.0029
1.0031 1.0031
1.0033 1.0033
1.0035 1.0036
1.0038 1.0038
1.0040 1.0040
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