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Setting Flow Rate
Setting Over-pressure Limit (P Max)
Solvent Compressibility Compensation
Refer to the Flow screen layout in Figure 7 for field orientation.
1. Press Flow and enter the numerical value for flow rate in µL/min,
including a decimal for fractional values.
2. Press Enter and then press Run or go to another setting.
Subsequent changes in flow rate require only entering the numerical
value and pressing Enter. Changes in flow may also be made by
using the
and
pressing Stop.
Figure 7 Flow Screen
Note: If pump head size is accidentally changed when using keyboard
control, reset it to Micro by repeatedly pressing the
1. Press P Max and enter numerical value for maximum desired over-
pressure limit.
2. Press Enter then press Run. If this P Max value is exceeded, the
pump will automatically shut down and prompt "Reset MAX Value?
Yes", then press Enter and reset a new P Max value and press
Enter. To resume operation without changing the P Max value,
press Enter and then press Run.
If the pump continues to reach P Max and shuts down, the cause needs
to be identified and corrected.
Note: At start-up, the pump sets the maximum pressure to a default
value which is 90% of the maximum specified upper pressure limit for the
pump head type.
Head Type
2 mL pump head
The 212-LC contains a library of solvents and their solvent
compressibility factors can be selected to automatically compensate for
changes in solvent compressibility. The solvents appear on the screen in
their abbreviated names as shown in the table on page 13. Relying on
the factory default value for solvent compressibility will result in ± 2.5%
accuracy for most solvents and pressures. Selecting the specific solvent
being used will result in a ± 1% accuracy or better for all pressures and
for all HPLC solvents.
12
keys. The pump can be stopped at any time by
Default Limit
Maximum Limit
5,400 psi
6,000 psi
key and Enter.
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