Edit Step Function; Edit Link Function; Edit Flow Rate; Edit Method File - Varian 212-LC Manual Del Usuario

Edit Step Function

The second field on the method screen is Step00. Enter the step size (%
step) or use the and keys to change the step size and press Enter.
Enter 0 if you do not want a step gradient. The step size you enter will
determine the percentage change in solvent B and the gradient time will
determine when each step will occur.
For example, if the step size is 10 and the method gradient is from 0 to
100%B in 30 minutes, the number of steps will be 10 (100/10) and the
step time will be 3 (30/10). In other words, the B composition will
increase 10% every 3 minutes. A programmed isocratic hold is required
to hold the composition at 100%B.

Edit Link Function

The step field is followed by the Link00 field. The link function allows you
to link the current method being edited to be linked to another method
file. Enter the desired link file number or use the keys to change the link
value and press Enter. When the pump has finished executing the last
line of the current method, the pump will begin to execute the first line of
the method file number that was entered into the link field.

Edit Flow Rate

The flow rate is entered in the Flow field. Enter the desired flow rate and
press Enter. This flow rate is equal to the combined flow of both pumps,
and will remain constant throughout a method run. Pump B will display
the flow rate for solvent B during the method run.

Edit Method File

The actual method file is a series of steps (T00 - T19) which include a
time (max 650.0 min. per step) and a percent composition (1 - 100) for
solvents A and B. When entering time, decimal points are not required.
For example, if you wish to set a time of 10 minutes, enter 100. Once the
percent composition for solvent A has been entered the solvent B
percent is entered automatically. Each numerical entry must be followed
by pressing Enter for that entry to be incorporated into the program. With
the cursor on a step (T00 - T19) the and keys can be used to scroll
between steps. The and keys can be used to scroll between the
step time, solvent composition, and step numbers.

Select Solvents

See page 12 for a complete explanation of solvent selection and
compressibility. Use the and keys to place the cursor at the
numeric field at the bottom left of the display and use the and keys
to select solvent. Follow the same procedure for Pump B.
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