Selecting A Solvent; Remote Control; Solvent Library - Varian 212-LC Manual Del Usuario

Solvent Library

Note: It is essential to degas the mobile phase to achieve maximum
accuracy and minimum pressure pulsations. If air is present in the pump
head, pulsation may occur. It may be necessary to run the pump for 10
to 30 minutes to remove residual air in the pump head.

Selecting a Solvent

1. Press Flow to edit the pump flow screen. A solvent library is listed on
page 13. Solvents are displayed in their abbreviated name with a
numerical compressibility factor. Use the
numerical value in the lower left corner of the display and the
keys to display a solvent to be selected. Press Enter. The pump
will now automatically compensate for the compressibility of the
selected solvent. For solvents not listed in the library, additional
compressibility values can be assigned to any of the labels
designated so13 to so29.
2. Use labels so13 to so29 for solvents whose compressibility values
are not in the pump library. Instructions on how to determine
compressibility factors (CF) for solvents other than those listed can
be found below.

Remote Control

The 212-LC Pump System can be controlled remotely in one of two
ways. External contact closures enable the system to be controlled by
most HPLC autosamplers. For instructions see page 18. The 212-LC
Pump System can also be controlled from a remote computer via the
RS232 interface using MS Workstation version 6.6 SP1 and above.
Abbreviation
H2O
ACN
MeOH
THF
STD
IPA
2PrOH
NHPTN
2BtOH
1BtOH
BtOAc
DMSO
Ace
so13-so29 User configurable
13
key to get to the
Solvent Name
Water
Acetonitrile
Methanol
Tetrahydrofuran
Factory default
Isopropyl Alcohol
2-Propanol
Heptane
Butanol - 2
Butanol - 1
n – Butyl Acetate
Dimethyl Sulfoxide
Acetone
and
Compressibility
Factor (CF)
100
180
185
160
125
167
167
200
180
190
170
167
195