MANUAL DE INSTRUCCIONES CODIGO 80055 REV B
MANUAL DE INSTRUCCIONES CODIGO 80055 REV D
16- Realizar el siguiente calculo:
J
.P. SELECTA s.a.
J
.P. SELECTA s.a.u.
OCT/2018
%Fiber =
%Fibra bruta / %Fiber =
Acetona
Acetone
Acetone
Crisol
Crucible
Crucible
Kitasatos
Kitasatos
3- FIBER COMPOSITION DETERMINATION BY VAN SOEST
METHOD.
- NEUTRAL DETERGENT FIBER (N.D.F):
The neutral detergent fiber is the insoluble residue in neutral detergent
(sodium sulphate lauril 3% pH 7). It includes Hemicellulose, Lignine,
Cellulose, Protein and Silice.Required reagents: -Neutral detergent
solution (N.D.S): Make a solution containing 30 g of sodium sulphate
lauril in 500 ml of distilled water and add 10 ml of 2-Etoxiethanol P.A..
Dissolve 18.61 g of EDTA Na
tetraborate P.A. in 250 ml of hot distilled water. Add this solution to the
above. Dissolve 4.56 g of disodium phosphate anhydre P.A. in 250
ml of hot distilled water. Add this solution to the above wich contains
all the other reagents. The final pH must be between 6.9 and 7.1, if it
is not, adjust with NaOH or HCl solution.
- Foam-suppressor such octanol
- Acetone
Required equipment:
Dosi-Fiber equipment
Scale
Vacuum pump
«Kitasatos» flask
Porous crucibles
Ctra. NII Km 585.1 Abrera 08630 (Barcelona) España
e-mail: jpselecta@sefes.es - http://www.sefes.es/selecta
Muffle furnace 500ºC
Oven at 150ºC
Vacuum dryer
Ctra. NII Km 585.1 Abrera 08630 (Barcelona) España
e-mail: jpselecta@sefes.es - http://www.sefes.es/selecta
19/02/99
(It can be modified without notice)
16- Carry out the following calculation:
W
- W
1
2
W
- W
1
W
0
W
H
O and 6.81 g of cahidrate sodium
2-
2
±0.1mg
(It can be modified without notice)
x 100
2
x 100
0
Agua
Water
Water
Trompa de vacío
Vacuum pump
Vacuum pump
Water
Agua
Water
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