J.P. SELECTA DOSI-FIBER 4 PLAZAS Manual De Instrucciones página 33

MANUAL DE INSTRUCCIONES CODIGO 80055 REV D
Do not make the cold extraction with acetone in the Dosi-fiber equipment
J
.P. SELECTA s.a.u.
OCT/2018
- ACID DETERGENT FIBER (A.D.F):
The acid detergent fiber is the insoluble residue in acid detergent
(cethyl trimethyl ammonium bromure). It includes lignine, cellulose,
and silice.
Required reagents:
- Acid detergent solution (A.D.S): To add 20 g of cethyl trimethyl
ammonium bromure to 1 l of sulphuric acid 1N.
- Acetone.
- Foam-suppressor such as octanol.
Required equipment:
Dosi-Fiber equipment.
Scale ±0.1mg.
Vacuum pump.
«Kitasatos» flask.
Porous crucibles.
Muffle furnace 500ºC.
Oven at 150ºC.
Vacuum dryer.
Procedure:
1- Weight and transfer ( precision ±0.1mg) of 1 to 1.5 mg sample
into a porous crucible. The quantity of sample is W
2- Place the crucible into the Dosi-fiber unit.
3- Ensure that the valve are in «OFF» position.
4- Add to each column 100 ml of acid detergent solution (A.D.S).
5- Open the cooling circuit and turn on the heating elements (power
at 90%)
6- Wait until it boils, reduce the power by 30% and leave boiling dur-
ing the extraction time (60 min.).
7. To stop heat. Open the vacuum circuit and place the valves in
«ABSORPTION». Fill with distilled water with the spray in each
column. Repeat this process 3 times.
Cold extraction with Acetone
8- Prepare the «Kitasatos» flask with the vacuum pump. Place the
crucible in the Kitasatos inlet and add acetone at the same time
that the vacuum circuit is absorbing toward the flask (like in the 1
and 2 methods). Repeat it 2 times.
Ctra. NII Km 585.1 Abrera 08630 (Barcelona) España
e-mail: jpselecta@sefes.es - http://www.sefes.es/selecta
(It can be modified without notice)
Tel (34 3) 770 08 77 Fax (34 3) 770 23 62
Pag.: 33
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