Puritan Diagnostics UniTranz-RT Manual Del Usuario página 6

Table 3 – Recovery of Mycoplasma and Ureaplasma
Dilution of
Organism
Neat Stock
Mycoplasma
hominis
Mycoplasma
pneumoniae
Ureaplasma
urealyticum
From each dilution, 100 µL were inoculated onto test swab tip followed by placement of the swab into the test
a
device containing 3 mL of transport medium
Average of triplicate tests (± standard deviation) performed on 100 µL of test device medium at each time point;
b
RT, room temperature; TNTC, too numerous to count, defined as 1,000 CFU for M. hominis and 2,000 CFU for M.
pneumoniae and U. urealyticum
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Occupationally Acquired Infections, 3
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Publication (CDC), 5
th
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American Society of Microbiology, Washington, D.C.
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6
Storage
Time
a
(Hours)
0
1:500 24
48
0
1:1000 24
48
0
Neat 24
48
0
1:10 24
48
0
1:500 24
48
0
1:1000 24
48
ed. CLSI, Wayne, PA.
rd
ed. Government Printing Office, Washington, D.C.
Incubation
Mean Viability of Test Organism Using Test
Time Prior to
(Puritan) Device: CFU Counts
Reading
(Days)
3
171 ± 42
3
160 ± 19
6
887 ± 334
6 416 ± 177
600 ± 303
5
811 ± 311
5 893 ± 486
611 ± 89
4°C
TNTC
TNTC
TNTC
171 ± 42
136 ± 9
TNTC
TNTC
TNTC 1116 ± 119
887 ± 334
275 ± 62
144 ± 53
TNTC
TNTC
TNTC
811 ± 311
775 ± 306
486 ± 134
ed. ASM, Washington, D.C.
nd
ed. Mosby, St. Louis, MO.
th
with SD
b
RT
TNTC
34 ± 5
75 ± 11
28 ± 7
9 ± 5
TNTC
TNTC
TNTC
TNTC
TNTC
ed.
th