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Bibliografía
General
Gallagher, S. R., and Smith, J. A., Electrophoretic separation
of proteins. In Current Protocols in Molecular Biology.
(Ausubel, F. A., eds.), OSC 10.2.1–10.2.21 (1991).
Hames, B. D., and Rickwood, D., Gel Electrophoresis of
Proteins: A Practical Approach: Second edition, City IRL
Press (1990).
Sambrook, J., and Russell, D.W., Molecular Cloning: A
Laboratory Manual. Cold Spring Harbor Laboratory,
Cold Spring Harbor, NY (2001).
Sasse, J., and Gallagher, S. R., Staining proteins in gels.
Current Protocols in Molecular Biology. (Ausubel, F. A., et
al., eds.), OSC 10.6.1–10.6.8 (1991).
SDS Polyacrylamide Gel Electrophoresis and Isoelectric
Focusing Handbook (80-6013-88), Hoefer, Inc. (2001).
No desnaturalizantes sistemas de gel
Reisfeld, R. A., et al., Acidic buffer system for resolution of
cationic proteins. Nature. 195, 281 (1962).
McLellan, T. Electrophoresis buffers for polyacrylamide gels
at various pH values. Anal. Biochem. 126, 94 (1982).
Hedrick, J. L. and Smith, A. J., Size and charge isomer
separation and estimation of molecular weights of proteins
by discontinuous gel electrophoresis. Arch. Biochem.
Biophys. 126, 155 (1968).
La desnaturalización sistemas de gel
Laemmli, U. K. Cleavage of structural proteins during the
assembly of the head of bacteriophage T. Nature. 227,
680–685 (1970).
Matsudaira, P. T. and Burgess, D. R., SDS microslab linear
gradient polyacrylamide gel electrophoresis. Anal.
Biochem. 87, 386–396 (1978).
Schreier, M. H., Erni, B., and Staehelin, T., Initiation
of mammalian protein synthesis. I. Purification and
characterization of seven initiation factors. J. Mol. Biol.
Nov; 116(4):727–753 (1977).
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