Amersham Pharmacia Biotech EPS 3501 XL Manual Del Usuario página 10

EPS 3501 XL - User Manual
Choosing step or gradient programming
Press SET ENTER to confirm step ( ) or use
ENTER.
Note that your choice of gradient or step programming applies for all phases within the program and
you will only be asked to choose one of them when programming the first phase.
Choosing step
electrophoresis will be controlled by one of these limiting values, which means that it is run at either
constant voltage, current or power. The EPS 3501 XL automatically switches over the controlling
parameter according to programmed limits and conductivity variations in the system. Thus the
controlling parameter can switch within a phase.
Table 1 and Fig. 4 illustrate a step program. The programming and running of this application are
shown as Figs. 3 and 7.
3 500
2 000
1 000
Fig. 4. Programming the voltage limiting profile in STEP mode. The parameters shown are the same as those
listed in Table 1.
Table 1. The parameters of a step program.
Phase Voltage
number (V)
1 1 000
2 500
3 2 000
4 3 500
Choosing gradient ( ), means that a voltage endpoint for the actual phase is programmed together with
current and power limiting values. A linear voltage gradient is made with zero (for the first phase) or the
programmed endpoint of the phase before (for the next phases) as starting point and the programmed
endpoint as endpoint. The electrophoresis will be controlled by this voltage gradient provided the
limiting current or power is not attained. The EPS 3501 XL thus automatically switches over the
controlling parameter according to the programmed limits and conductivity variations in the system.
8
means that voltage, current and power limiting values are programmed. The
Phase 1
1 000 Vh
500
Current Power
(mA) (W)
10 10
10 5
20 15
30 25
/ keys to choose gradient ( ). Confirm with SET
Phase 3
1 000 Vh
Phase
2
120 Vh
1
Volthours
(Vh)
1 000
120
1 000
1 500
Phase 4
1 500 Vh
2
Time (h)