Introduction; Safety Information; Safety Precautions - Amersham Pharmacia Biotech EPS 3501 XL Manual Del Usuario

EPS 3501 XL - User Manual

1. Introduction

The Amersham Pharmacia Biotech Electrophoresis Power Supply EPS 3501 XL is a high quality, high
precision and safe power supply for electrophoresis applications that require advanced programming and
high voltage.
EPS 3501 XL is primarily designed for techniques using programming in several phases and/or voltage
gradients:
• 2-D (Two dimensional) electrophoresis using Immobiline™
• IEF (Isoelectric focusing) using Immobiline
EPS 3501 XL is also suitable for:
• DNA sequencing
• SDS-PAGE (Polyacrylamide Gel Electrophoresis)
• Native PAGE
• Agarose electrophoresis
• Electroblotting
• DNA pulsed field electrophoresis
Nine programs each with up to nine phases can be saved. Limiting values for voltage, current and power
as well as voltage gradients can be programmed for precise control of the electrophoresis. The
EPS 3501 XL automatically switches over the controlling parameter according to programmed limits and
gradients and conductivity variations in the system.
Two electrophoresis units can be connected to the EPS 3501 XL and run with the same programmed
method at one time.

2. Safety information

Extreme caution should be exercised in the operation of this instrument as it can develop sufficient
voltage and current to produce a lethal shock.

2.1 Safety precautions

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To avoid any risk of injury, the instrument should only be operated by properly trained personnel and
always in accordance with the instructions provided.
Read this entire manual before using this power supply.
1. This instrument is designed for indoor use only.
2. The instrument must always be used with the protective earth lead of the power cord correctly
grounded to earth at the mains outlet.
3. To permit sufficient cooling, ensure that the vents in the rear and sides of the instrument are not
covered.
4. Do not operate the instrument in extreme humidity (above 95%). Avoid condensation by letting the
unit equilibrate to ambient temperature when taking the power supply from a colder to a warmer
environment.
5. Keep the instrument dry and clean. Wipe regularly with a soft damp cloth. Let the power supply dry
completely before use. If wetted, unplug the power supply until the instrument is dry.
6. Use only undamaged electrical wire and equipment approved for the voltages you will use. High
voltage wires must meet the requirements of the IEC 1010-2-031:1993 electrical standard.
Any electrophoresis equipment connected to the power supply should meet the requirements of the
IEC 1010-1:1993.
7. Note that the output is connected to the chassis/reference earth.
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