Amersham Pharmacia Biotech EPS 3501 XL Manual Del Usuario página 11

3 500
Phase 1
1 Vh
500
1
Fig 5. Programming the voltage limiting profile in GRADIENT mode. The parameters shown are the same as
those listed in Table 2.
To illustrate voltage gradient programming, the programming for the IEF part of a 2-D electrophoresis
with Immobiline DryStrip™ is shown in Table 2 and Fig. 5. Note that the first phase is a very steep
gradient to reach the 500 V start level (0 - 500 V, within 1 Vh). The next phase is actually a step since
the endpoint for phase 2 is 500 V which is the same as the endpoint for phase 1. Phase 3 is the "real"
gradient, the voltage is changed from 500 to 3 500 V in 5 hours. The last phase is a step again, the
endpoint voltage is the same as for the phase before and the voltage will remain on 3 500 V for 9.5
hours.
Table 2. The parameters of a gradient program.
Phase Voltage Current
number (V) (mA)
1 500 1
2 500 1
3 3 500 1
4 3 500 1
* The ramping from 0 to 500 V should be done as quickly as possible. The smallest possible time that can be set is 1 minute and the smallest
possible Vh that can be set is 1 Vh. Vh was chosen for this program as breakpoint unit.
Choose phase number
Choose phase number with
number is 1. If the first phase has been programmed and the question "Last phase ?" is answered by NO,
the default number is 2 and so on.
Setting voltage, current and power
The display will now flash for the set voltage limit (step mode) or voltage endpoint (gradient mode).
Using the / keys, select the voltage limit or voltage endpoint desired for the run. Confirm with SET
ENTER.
Phase 3
Phase 2
10 000 Vh
2
2 500 Vh
5
Power Time Volthours
(W) (h)
5 0:01 *
5 5
5 5 10 000
5 9.5 33 250
/ and confirm with SET ENTER. For a new program the default phase
EPS 3501 XL - User Manual
3
Phase 4
33 250 Vh
10
(Vh)
1 *
2 500
4
19.5
Time (h)
9