Amersham Pharmacia Biotech EPS 3501 XL Manual Del Usuario página 14

EPS 3501 XL - User Manual
1. Choose Program Number (1→2)
2. Confirm Step or Gradient.
(Step)
3. Choose Phase number (2) and
enter INSERT/DELETE
4. Choose INSERT or DELETE
5. Program
SET:
a new
0 V
phase 2.
Programming point 4
elapsed time, volthours or milliamperehours by
underlined.
A light emitting diode shows when voltage is applied (HV on).
If no current is displayed or if "HALT: Low start current" is shown, please check the electrical
connections to the electrophoresis equipment.
Pausing
You can interrupt the electrophoresis for sample loading and/or changing the program by pressing
PAUSE CONTINUE. Voltage will no longer be supplied, the "HV on" LED goes off, and you may safely
load your samples. The display shows the status of the run when PAUSE CONTINUE was pressed.
Switch between time, integrated voltage and integrated current for the phase running when PAUSE
CONTINUE was pressed with
When sample loading is complete, press either PAUSE CONTINUE again or RUN to continue the run
from where it was interrupted.
Editing a running program when in PAUSE
When in the PAUSE mode you can also press SET ENTER to make changes in the program. This mode is
called the P-SET mode. When the P-SET mode is entered you can make changes as described in the
12
SET: 1 1
500 V 1 mA
Select by
SET: 2 1
step/GRADIENT
set
Confirm by
enter
SET: 2 1
1000 V 120 mA
Select by
Insert Phase 2
INS/del
Select by
INSERT
2 2 00:00 h
0 mA 0 W
Fig. 6. Inserting and deleting a phase in a program.
/ .
A
1 Vh
5 W
set
Confirm by
enter
1000 Vh
10 W
Insert
Enter by
delete
set
Confirm by
enter
DELETE
SET: 2 2
2000 V 20 mA
/ . The parameter controlling the electrophoresis is
5. Back to new
1000 Vh
Phase 2 start
15 W
position.
Old Phase 2
deleted.