Limitations Of Use - DRG EIA-2395 Instrucciones De Uso

9.5 Recovery
Samples have been spiked by adding Leptin solutions with known concentrations.
The % recovery has been calculated by multiplication of the ratio of the measurements and the expected values with 100
(expected value = (endogenous Leptin + added Leptin) / 2; because of a 1:2 dilution of serum with spike material).
Concentration (ng/mL)
Average Recovery
Range of Recovery (%)
9.6 Linearity
Concentration (ng/mL)
Average Recovery
Range of Recovery (%)
10

LIMITATIONS OF USE

Reliable and reproducible results will be obtained when the assay procedure is performed with a complete understanding
of the package insert instruction and with adherence to good laboratory practice.
Any improper handling of samples or modification of this test might influence the results.
10.1 Interfering Substances
Haemoglobin (up to 4 mg/mL), Bilirubin (up to 0.5 mg/mL) and Triglyceride (up to 30 mg/mL) have no influence on the
assay results.
10.2 Drug Interferences
Until today no substances (drugs) are known to us, which have an influence to the measurement of Leptin in a sample.
10.3 High-Dose-Hook Effect
Hook effect was not observed in this test up to a concentration of 500 ng/mL of Leptin.
Version 10.0 2017/05 - vk
Leptin Sandwich ELISA EIA-2395
Sample 1 Sample 2
4.6 21.4
88.8 97.0
from 86.8 90.6
to 93.1 102.1
Sample 1 Sample 2
4.6 21.4
93.2 92.7
from 85.1 86.2
to 107,5 103.1
-
10 -
Sample 3
9.6
94.8
84.0
106.0
Sample 3
9.6
104.7
88.0
114.3