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6
Sample collection and preparation
Sample material, sample volume and collection
Sample
Sample
material
volume
Fingertip
sample
20 μl
(whole
blood)
Antico-
agulated
20 μl
whole
blood
20 μl or
Plasma
12 μl *
20 μl or
Serum
12 μl *
* If you wish to use the 12 μl sample volume and achieve a wider measurement range,
change the volume setting of the QuikRead go instrument. Consult the instrument instruc-
tions for use: Settings → Measurement flow → Test parameters.
Sample dilution
Sample material Instructions
Whole blood
Plasma/
serum
Sample storage
Sample material
Fingertip blood (whole
blood)
Anticoagulated whole blood 2...8 °C for 3 days
Plasma
Serum
Sample (whole blood,
plasma, serum) in buffer
Allow the samples to reach room temperature (18...25 °C) before testing. Frozen samples
must be thawed out, allowed to reach room temperature and mixed carefully prior to testing.
If the thawed plasma or serum samples contain clots, they must be centrifuged. Samples
should not be repeatedly frozen and thawed.
7
Procedure
Materials required but not supplied
Material
QuikRead go
Instrument with
®
software version 4.1 or newer
QuikRead go
CRP Control
®
QuikRead go
CRP Control High
®
QuikRead
CRP Control
®
QuikRead go
CRP Control High
®
QuikRead go
Hb Control
®
Fingertip lancets
Assay procedure
Open the foil pouch protecting the cuvette rack and mark the opening date on the cuvette
rack.
Sample collection (Fig. 1–5)
Glass capillaries (20 μl) and plungers are recommended for adding the sample into a
prefilled cuvette.
1
A prefilled cuvette must reach room temperature (18...25 ºC) before use. This will take
15 minutes for an individual refrigerated (2...8 ºC) prefilled cuvette. Do not touch the
clear flat surfaces in the lower part of the cuvette (the optical part). Remove the foil cover
from the cuvette. Beware of splashing the liquid. Condensed buffer on the foil cover has
no effect on results. The test must be performed within two (2) hours from opening the
cuvette. The liquid surface should lie between the two lines marked on the cuvette.
2
Insert the plunger into the capillary at the end with the blue stripe.
3
Fill the capillary with the sample up to the white stopper (20 µl). Ensure that there are no
air bubbles in the capillary. Use a soft cloth or tissue to wipe away any residual sample
from the outside of the capillary.
4
Place the sample capillary into the buffer solution in the cuvette and dispense by
pressing down the plunger. Make sure that the capillary is completely empty.
5
Close the cuvette tightly with a CRP reagent cap. Do not press down the blue inner part
of the reagent cap. After the sample has been added to the buffer, the solution is stable
for two (2) hours. Keep the cuvette in an upright position, do not shake.
Analysing the sample (Fig. 6–8)
For full instructions, see QuikRead go instrument instructions for use. The display will guide
you through the test.
6
On the QuikRead go instrument display, select Measure.
7
Place the cuvette in the instrument with the barcode facing you, as shown in Figure
7. The instrument recognises the sample type. The display shows how the measuring
progresses. The instrument first measures the sample blank and then the CRP and Hb
concentrations within one minute.
8
The result appears on the display when the measurement is completed and the cuvette
automatically rises from the measurement well.
Analysing the control (Fig. 7–8)
Precise instructions on the use of controls are provided in the package insert of the control
vials. Analyse the control samples as you would clinical samples, but select Quality Control
on the instrument display (Fig. 7–8). Select the control that matches the test you are using
(CRP or Hb). The result will be saved as a control measurement.
8
Quality control
Regular use of QuikRead go CRP Control (Cat. no. 153764) and/or QuikRead go
CRP Control High (Cat. no. 153763) and QuikRead go Hb Control (Cat. no. 141154) is
recommended. Alternatively QuikRead CRP Control (Cat. no. 68296) and/or QuikRead go
CRP Control High (Cat. no. 137071) can be used for quality control.
QuikRead CRP controls: All QuikRead CRP controls are ready-to-use. The control values
have been determined for the QuikRead go instrument, and the control is applied using the
same procedure as for a plasma/serum sample. The sample volume is 20 μl. If you wish to
use the 12 μl sample volume, change the volume setting (plasma/serum) of the QuikRead
go instrument. Consult the instrument instructions for use: Settings → Measurement flow
→ Test parameters.
Other commercially available CRP controls: Handle and use the control as instructed
and measure it as you would the QuikRead CRP controls. Acceptance limits for the control
value must be defined with the QuikRead go instrument. The blanking process might
not succeed if you use a control that contains artificial red blood cells as these might not
haemolyse normally.
QuikRead go Hb control: QuikRead go Hb Control is a ready-to-use control.
The control values have been determined for the QuikRead go instrument, and the control
is used as whole blood sample. The sample volume is 20 μl.
Other commercially available Hb controls: Handle and use controls as instructed.
Acceptance limits for the control value must be defined with the QuikRead go instrument.
The blanking process might not succeed if you use a control that contains artificial red
blood cells as these might not haemolyse normally. Controls in which the hemoglobin is
present in a form other than oxyhemoglobin, or which contain compounds which may modify
hemoglobin, might yield results that are not aligned with those obtained by other methods.
Obtainable
result
CRP and
hemoglobin
CRP and
hemoglobin
CRP
CRP
Do not dilute whole blood samples.
Plasma or serum samples may be diluted with a 0.9% NaCl solution before
adding the sample to the cuvette.
The recommended dilution ratio is 1+3. Multiply the obtained result by four (4).
Short-term storage
Max. 15 minutes in the
heparinised capillary
2...8 °C for 7 days
2...8 °C for 7 days
18...25 °C for 2 hours
Sample collection
Prick the clean and dry finger with a lancet and discard the
first drop. Wipe the finger and collect 20 μl of blood from
the second drop in a heparinised capillary.
Use a venous blood sample collected in a tube containing
heparin or EDTA. Mix whole blood by inverting the tube
several times and take 20 µl of the sample with a capil-
lary or pipette.
Use EDTA/heparin plasma. Avoid clearly haemolysed
samples. Mild haemolysis will not affect the test result.
Avoid clearly haemolysed samples. Mild haemolysis will
not affect the test result.
Cat. no.
Additional info
133893
Recommended for quality control
153764
Recommended for quality control
153763
Alternative quality control
68296
Alternative quality control
137071
Recommended for quality control
141154
Long-term storage
Do not store
Separate plasma and store at
below –20 °C
Below –20 °C
Below –20 °C
Do not store
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