3M L25PNT Instrucciones Del Producto página 4

Instructions for Use
Follow all instructions carefully. Failure to do so may lead to inaccurate results. Ensure that all 3M Peanut Protein Rapid
Kit components are at ambient temperature (20-25°C) prior to use.
Sample Analysis
1. CIP Final Rinse Water Samples
1.1 Label one microcentrifuge tube for each CIP sample.
1.2 Add 800 μL of 3M Extraction Buffer to a labeled microcentrifuge tube.
1.3 Add 200 μL of CIP final rinse water sample. Shake vigorously or vortex for 15 seconds to mix thoroughly to
obtain an extracted sample.
Note: The pH of extracted sample should be between 5 and 10. Proceed to Troubleshooting section for
additional information.
1.4 Remove one 3M Peanut Protein LFD from package and place on a clean, dry, flat surface.
1.5 Transfer 100 μL of the extracted sample prepared in 1.3 using a clean pipette or pipette tip and apply it to
the sample well on the 3M Peanut Protein LFD. Start the timer for 11 ± 1 minutes. Proceed to Interpretation of
Results section.
2. Environmental Swab Samples
2.1 Label one microcentrifuge tube for each Environmental Swab Sample.
2.2 Add 500 μL of 3M Extraction Buffer into a labeled microcentrifuge tube.
2.3 Take a clean swab and dip the entire tip into the microcentrifuge tube wetting the tip with 3M Extraction
Buffer. Gently express excess liquid from tip by pressing swab tip lightly on the inside of the tube.
2.4 Take wetted swab and survey a 10 X 10 cm surface area maintaining the swab at a 30° angle with the surface.
Rub the swab slowly and thoroughly over the surface area. Rub the swab three times over this surface,
reversing direction between alternating strokes.
2.5 Take swab and insert it back into the pre-labeled tube and swirl swab several times to release any residues
that might be on the surface of swab into the 3M Extraction Buffer. Break off swab tip in tube, cap tightly and
mix well to obtain an extracted sample.
2.6 Remove one 3M Peanut Protein LFD from the package and place on a clean, dry, flat surface.
2.7 Transfer 100 μL of the extracted sample prepared in 2.5 using a clean pipette or pipette tip and apply it to
the sample well on the 3M Peanut Protein LFD. Start the timer for 11 ± 1 minutes. Proceed to Interpretation of
Results section.
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