Simultaneous Observation Phase Contrast + Fluorescence (B-383Fl); Maintenance - Optica B-380 Serie Manual De Instrucciones

15. Simultaneous observation Phase Contrast + Fluorescence (B-383FL)

• This microscope allows observation in transmitted light Phase contrast in combination with reflected
light Fluorescence. Samples with rapid decay must first be observed in Fluorescence and then in
Phase Contrast. The combined observation allows you to easily identify some areas of the sample
that emit fluorescence.
1. Turn on the power supply for the HBO fluorescent bulb and wait 5 minutes before the arc stabilizes.
2. Move the filter selector to an empty position.
3. Insert the desired PH lens and rotate the phase contrast condenser turret to the position containing the
corresponding phase ring.
4. Focus the sample.
5. Adjust the light intensity of the transmitted light.
6. Move the fluorescence filter selector to the desired position.
7. To obtain the proper observation of the sample, adjust the light intensity of the transmitted light to modulate
the intensity of the fluorescence with that of the phase contrast.

16. Maintenance

Microscopy environment
This microscope is recommended to be used in a clean, dry and shock free environment with a temperature of
5°-40°C and a maximum relative humidity of 75 % (non condensing). Use a dehumidifier if needed.
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no
moving parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn off the light immediately, cover the microscope with the included dust-cover, and
keep it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region matches with
the operation voltage of the equipment and that the lamp switch is in off position.
• Users should observe all safety regulations of the region. The equipment has acquired the CE safety
label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try first to: use compressed air.
• If that is not sufficient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a final option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
Note: ethanol and ether are highly flammable liquids. Do not use them near a heat source, near sparks or
near electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the
optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
For the best results, use the OPTIKA cleaning kit (see catalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.
Page 33