Troubleshooting - Optica B-380 Serie Manual De Instrucciones

17. Troubleshooting

Review the information in the table below to troubleshoot operating problems.
PROBLEM
I. Optical Section:
LED operates, but field of view re-
mains dark.
Field of view is obscured or not
evenly illuminated
Dirt or dust is visible in the field of
view.
Image looks double
Visibility is poor.
· Image is not clear.
· Contrast is poor.
· Details are indistinct.
· Image glares
One side of the image is unfocused
CAUSE
Power supply is unplugged.
Brightness is too low
Fluorescence filter selector is
not in a click stop
Fluorescence shutter is closed
Fluorescence filter is not suit-
able for the specimen
Revolving nosepiece is not cor-
rectly engaged.
The turret of the phase contrast
condenser is in an incorrect
position
Dirt/dust on the specimen
Dirt/dust on the eyepieces
Aperture iris diaphragm is
stopped down too far.
The condenser is not well cen-
tered or it is in a wrong height
Revolving nosepiece is in an
incorrect position
Aperture iris diaphragm is too
closed or too open.
Dust or dirt on lenses (conden-
ser, objectives, eyepieces and
slide)
For transmitted light observa-
tion, the coverglass thickness
must not exceed 0.17mm
For phase contrast observation,
a brightfield objective is used
instead a phase contrast one
Phase rings of objective and
condenser are not well cente-
red
Objective in use is not compati-
ble with condenser phase ring
Focus is not even
Revolving nosepiece is in an
incorrect position
Slide is mounted not in a flat
position (tilted)
Poor quality of the glass slide
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SOLUTION
Connect
Set brightness to a proper level
Move the selector to a click stop
Open the shutter
Use a suitable filter
Make sure that the revolving nosepiece
clicks properly into place.
Move the turret to a click stop
Clean the specimen
Clean the eyepieces
Open aperture iris diaphragm.
Set the condenser according to Koehler
settings.
Move the nosepiece to a click stop
Adjust aperture iris diaphragm.
Clean thoroughly.
Use a coverglass with thickness 0.17mm
Usa a phase contrast objective
Operate on centering screws
Use a compatible objective
Slide holder is not flat. Move the specimen
to a flat position.
Move the nosepiece to a click stop
Place the specimen in a flat position on the
stage
Use a glass slide with higher quality