3M Listeria MDAL96 Manual Del Usuario página 8

PREPARATION OF THE 3M™ MOLECULAR DETECTION SPEED LOADER TRAY
1. Wet a cloth with a 1-5% (v:v in water) household bleach solution and wipe the 3M™ Molecular Detection Speed Loader Tray.
2. Rinse the 3M Molecular Detection Speed Loader Tray with water.
3. Use a disposable towel to wipe the 3M Molecular Detection Speed Loader Tray dry.
4. Ensure the 3M Molecular Detection Speed Loader Tray is dry before use.
PREPARATION OF THE 3M™ MOLECULAR DETECTION CHILL BLOCK INSERT
Before using the 3M™ Molecular Detection Chill Block Insert, ensure it has been stored on the 3M™ Molecular Detection Chill Block Tray in the
freezer (-10 to -20°C) for a minimum of 2 hours before use. When removing the 3M Molecular Detection Chill Block Insert from the freezer for use,
remove it and the 3M Molecular Detection Chill Block Tray together. Use the 3M Molecular Detection Chill Block Insert /3M Molecular Detection Chill
Block Tray within 20 minutes.
PREPARATION OF THE 3M™ MOLECULAR DETECTION HEAT BLOCK INSERT
Place the 3M™ Molecular Detection Heat Block Insert in a dry double block heater unit. Turn on the dry block heater unit and set the temperature to
allow the 3M Molecular Detection Heat Block Insert to reach and maintain a temperature of 100 ±1°C.
NOTE: Depending on the heater unit, allow approximately 30 minutes for the 3M Molecular Detection Heat Block Insert to reach temperature.
Using an appropriate, calibrated thermometer (e.g., a partial immersion thermometer, digital thermocouple thermometer, not a total immersion
thermometer) placed in the designated location, verify that the 3M Molecular Detection Heat Block Insert is at 100 ±1°C.
PREPARATION OF THE 3M™ MOLECULAR DETECTION INSTRUMENT
1. Launch the 3M™ Molecular Detection Software and log in.
2. Turn on the 3M Molecular Detection Instrument.
3. Create or edit a run with data for each sample. Refer to the 3M Molecular Detection System User Manual for details.
NOTE: The 3M Molecular Detection Instrument must reach and maintain temperature of 60°C before inserting the 3M Molecular Detection Speed
Loader Tray with reaction tubes. This heating step takes approximately 20 minutes and is indicated by an ORANGE light on the instrument's status
bar. When the instrument is ready to start a run, the status bar will turn GREEN.
LYSIS
1. Allow the lysis solution (LS) tubes to warm up to room temperature (20-25°C) by setting the rack on the laboratory bench for at least 2 hours
2. Remove the enrichment broth from the incubator and gently agitate the contents.
3. One LS tube is required for each sample and the Negative Control (NC) sample.
3.1 LS tube strips can be cut to desired LS tube number. Select the number of individual LS tubes or 8-tube strips needed. Place the LS tubes in
an empty rack.
3.2 To avoid cross-contamination, decap one LS tubes strip at a time and use a new pipette tip for each transfer step.
4. Transfer enriched sample to LS tubes as described below:
4.1 Use the 3M™ Molecular Detection Cap/Decap Tool-Lysis to decap one LS tube strip - one strip at a time. Set the tool with cap attached aside
on a clean surface.
4.2 Transfer 20 µL of sample into a LS tube.
4.3 Repeat step 4.2 until each individual sample has been added to a corresponding LS tube in the strip.
4.4 Use the 3M Molecular Detection Cap/Decap Tool-Lysis to re-cap the LS tube strip. Use the rounded side of the tool to apply pressure in a back
and forth motion ensuring that the cap is tightly applied.
4.5 Repeat steps 4.1 to 4.4 as needed, for the number of samples to be tested.
4.6 When all samples have been transferred, transfer 20 µL of NC into a LS tube. Use the 3M Molecular Detection Cap/Decap Tool-Lysis tool to
re-cap the LS tube.
4.7 Cover the rack of LS tubes with the rack lid and firmly invert 3-5 times to mix. Suspension has to flow freely inside the tube.
Transfer each enriched sample into an individual LS tube first. Transfer the NC last.
20 µL
30 µL
LS tube
LS tube
LS rack
LS rack
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