4. Use the CT to focus the condenser phase ring (bright)
the objective phase ring (dark)
ring's image is not sharp, adjust the CT head until you can
see a clear image of the phase ring. (Fig. 40)
5. Adjust the bolts of the two centering holes in the phase con-
trast slider using the provided Allen wrenches
bright ring and the dark ring match. (Fig. 41)
6. The 4x and the 10X phase contrast objectives use the same
ring on the phase contrast slider. The coincidence of the
phase ring center and the phase contrast center must be
verified with both objectives. (Fig. 42)
•
If the phase ring is incorrectly centered, the contrast will
be severely impaired.
•
The phase ring may need recentering during and after
observation of very thick specimens.
•
The phase ring may show an apparent misalignment if
the specimen is not flat.
①
②
image. If the bright phase
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until the
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①
②
③
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ig. 40
F ig. 41
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ig. 41
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ig. 42