Optika Italy IM Serie Manual De Instrucciones página 29

14. Use of the microscope in RPC (optional)
Relief phase contrast (RPC) is a modification of conventional phase contrast that leads to visible improvements in image
quality in optical microscopy. Specifically, the following parameters can be improved: contrast, focal depth, sharpness,
three-dimensionality, flatness, and halo artifacts. These effects can be achieved when the phase rings of the condenser are
replaced by slit rings.
Similar to phase contrast observation, RPC observation requires the use of a slider containing slit phase rings and dedicated
RPC objectives.
The use of the slider and objective are identical to those for phase contrast.
14.1 Installing the RPC slider
1. Insert the slider into the lower slot of the illumination system,
printed face up. (Fig. 43)
2. Pull the slider into the desired position, until it arrives to the
click stop.
3. When in RPC observation, keep the aperture diaphragm ad-
①
justment lever on the "O" (open) position.
14.2 RPC slider
• Two sliders are available for the use with different objectives.
• One slider is dedicated to 4X objective (Fig. 44) and another
is for 10X/20X/40X objectives. (Fig. 45)
• Both have an empty hole and a RPC ring.
SLIDER
MEANING
POSITION
EMPTY
empty hole
4x
RPC ring 4x
RPC ring
10x/20x/40x
10x/20x/40x
APPLICATION
brightfield
②
observation
RPC observation with
③
4x objective
RPC observation with
10x, 20x and 40x
④
objectives
①
②
②
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F ig. 43 F ig. 43
③
F ig. 44 F ig. 44
④
F ig. 45 F ig. 45