Optika Italy IM Serie Manual De Instrucciones página 31

15. Simultaneous observation in Phase Contrast / RPC + Fluorescence
• Fluorescence models allow observation in transmitted light Phase contrast or RPC in combination with
reflected light Fluorescence. Samples with rapid decay must first be observed in Fluorescence and then in
Phase Contrast / RPC. The combined observation allows you to easily identify some areas of the sample that
emit fluorescence.
1. Turn on the microscope main switch.
2. Move the filter selector to an empty position or, if the filter holder is full, to the position containing the UV filter.
3. Insert the desired PH / RPC objective and move the phase contrast / RPC slider to the position containing the
corresponding phase ring.
4. Focus the sample.
5. Adjust the light intensity of the transmitted light.
6. Move the fluorescence filter selector to the desired position.
7. Adjust the light intensity of the reflected light.
8. To obtain the proper observation of the sample, adjust the light intensity of the transmitted light to modulate the intensity
of the fluorescence with the one of the phase contrast / RPC.
16. Use of camera (IM-300LD4D)
1. Tap the icon for the ProView software (or double-click the icon with the mouse). The software starts.
2. In the "Camera List" panel, the "C-P6" item is shown.
3. Tap on the C-P6 item (or click with the mouse): the live image will be displayed on the main window of the software.
4. Adjust the camera parameters by acting on the exposure time ("Exposure and Gain" panel) and the white balance
("White Balance" panel).
5. Once the first adjustments have been made, you can operate normally.
• The software's User Manual is available in PDF format within the application itself and can be opened using the "F1"
function key. The manual contains all the operating instructions for using the camera and for the various functions of
the software.
• You must have Acrobat Reader installed to view the manual.
17. Micrometric Slide M-005
(1mm/100div. for biological microscopes / 10mm/100div. for stereomicroscopes)
1 DIV=0.01mm
For biological microscopes calibration
Micrometric slide, 26x76mm, with 2 scales
Page 31
1 DIV=0.1mm
For stereo microscopes calibration