Test Procedure - Abbott NycoCard HbA1c Manual Del Usuario

TEST PROCEDURE

Important procedural notes!
• Do not interchange components from different
kits or kit lots.
• Bring the R1/reagent to room temperature
(20-25°C) before use.
• Do not touch the membrane with the pipette tip.
• Change the pipette tip between each
pipetting step.
• The procedure steps should be performed
successively without any interruptions.
Test procedure (illustrations on page 65)
1
Prepare sample
Add 5 μL whole blood to the test tube with R1/reagent. Mix well. Leave the tube for
minimum 2 minutes, maximum 3 minutes. Use a timer.
Note! Equilibrate the R1/reagent to room temperature (20-25°C) before use.
2
Apply sample
Remix to obtain a homogenous suspension. Apply 25 μL of the mixture to a TD/test device.
Hold the pipette approx. 0.5 cm above the test well and empty the pipette quickly in the middle
of the test well. Allow the mixture to soak completely into the membrane. Wait for minimum
10 seconds.
Note! Avoid air bubbles.
3
Apply R2/washing solution
Apply 25 μL R2/washing solution to the TD/test device. Allow the reagent to soak completely
into the membrane. Wait for minimum 10 seconds.
Note! Avoid air bubbles.
4
Read the test result
Read the test result within 5 minutes using the NycoCard Reader II. Follow the NycoCard
Reader II Instruction Manual.
Sample material
Capillary and venous whole blood. Avoid
measuring hemolysed samples (see "Limitations
of the test"). Mix venous samples well by
inverting the tube 8-10 times before collecting
a sample.
NycoCard HbA1c
7