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Methodology
Guidelines
A78310250ES 5.1ª Edición
When first setting up the Cytospin for use, it is helpful to
establish a standard procedure, i.e. the amount of specimen
per slide, rpm and minutes centrifuged, and maximum
number of white blood cells per litre and red blood cells per
litre, above which dilutions would be necessary.
1 Determine the red cell and white cell count of the
sample according to established methods.
2 Using a 'standard' amount of specimen, (e.g. 5 drops or
approximately 0.25 ml) make serial dilutions of a highly
cellular specimen to determine the maximum number of
white blood cells and red blood cells that can be present
in a specimen before dilutions are necessary. Some tests
may require more cellular slides than others.
3 Experiment with a range of speeds to see which gives the
most desirable morphology for the procedure involved.
Establish the minimum number of minutes required to
spin the entire standard amount of specimen onto the
slide.
4 The following is an example of a standard procedure for
preparing slides for a white cell differential with Wright
stain:
Use 5 drops of specimen per Cytospin chamber. Dilute
sample to obtain a white cell count less than 0.5 x 10
and a red cell count less than 0.005 x 10
at 700 rpm for 5 minutes with medium acceleration.
Manual de operaciones de la Cytospin 4
Methodology Guidelines
9
/ l. Centrifuge
12
/ l
105
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