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Methodology Guidelines
1 drop
4 drops
12 drops
Cells / 40x field as a guideline to the number of drops of cell suspension
/ Cytospin sample chamber
92
Manual de operaciones de la Cytospin 4
2 drops
3 drops
5 drops
6 drops
20 drops
Count the cells seen in the field. It is not necessary to
count every cell - an approximation will do. Refer to the
illustration Cells / 40x field as a guideline to the number
of drops of cell suspension / Cytospin sample chamber. This
count can be used to estimate the number of drops of the
cell suspension required for a Cytospin preparation. To
determine the number of cells being used, multiply the
number of cells counted by 38. Divide the number of cells
counted into 60. The quotient equals the number of drops
that should be added to the Cytospin sample chamber,
though the total volume should not exceed the 0.5 ml
capacity of the chamber. This gives the total number of cells
applied to the Cytospin funnel for each drop of suspension
used. While this technique for estimation of cell number is
an approximation only, it does provide an excellent control
of Cytospin preparations.
A second method of cell number determination is by manual
The large circles represent cells
in one drop of cell suspension,
either of unprocessed cell specimen
or preferably of centrifuged cell
concentrate, that have been spread
under a 24 x 50 mm cover glass
and viewed under a 40x objective.
Although drawn smaller than they
appear microscopically, the cell and
field diameters are proportional to
one another at a 50:1 ratio. Simply
match the microscopic field with its
closest counterpart here and use the
number of drops so indicated.
A78310250ES 5.1ª Edición
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