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Methodology Guidelines
106
Manual de operaciones de la Cytospin 4
Table 2 Dilution Chart for WBC Dilution
WBC Count
(x 10
/ litre)
9
0 - 0.05
0.05 - 0.1
0.1 - 0.15
0.15 - 0.2
0.2 - 0.25
0.25 - 0.3
If the red cell count which results from the above WBC
dilution is greater than 0.005 x 10
further dilution necessary to bring the red cell count
below 0.005 x 10
fact that the white cell count may be very low.
5 Use a disposable EZ Single Cytofunnel.
6 Always place the EZ Cytofunnel assembly into the
Cytospin head before adding anything to the sample
chamber. Check that the assembly pivots freely in the
metal support plate.
7 Use coated slides to increase the adhesion of the cells to
the slides. Cap the sample chambers.
8 Lock the lid onto the sealed head, transfer the sealed
head to the Cytospin, and close the lid. Never snap the
lid of the Cytospin head down onto the bowl while the
assembly is sitting on the drive shaft, as this may damage
the shaft.
9 Program the Cytospin to the standard number of rpm
and minutes and start the unit. Fragile cells may require
a lower rpm setting and low acceleration to maintain
morphology.
Dilution
None
1/2
1/3
1/4
1/5
1/6
12
/ l. Use this dilution regardless of the
12
A78310250ES 5.1ª Edición
/ l, calculate the
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