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Methodology Guidelines
Selecting Time,
Speed and
Acceleration
96
Manual de operaciones de la Cytospin 4
careful during loading not to forcibly inject the sample into
the sample chamber. The sample should be eased into the
sample chamber slowly, allowing ample opportunity for air
to be displaced by the sample.
For concentrated cell suspensions which require only one or
two drops of sample to obtain the correct cell concentration,
it may be sometimes necessary to add additional diluent to
bring the total volume in the sample chamber up to 0.5 ml.
This addition can be done in the chamber as the samples
are loaded. However, this requires care to avoid forcing
sample into the slide / filter area and it is recommended that
'thick' specimens are diluted prior to being loaded into the
Cytospin.
During loading of the sample chamber, the sample should
be deposited directly in the bottom of the sample chamber.
Avoid dripping the sample down the side of the sample
chamber. Should sample be deposited on the walls of the
chamber, rinse down with a drop of diluent. The object is to
ensure that the entire sample is in the bottom of the sample
chamber.
The speed of operation of the Cytospin is dependent on the
size of the cells or particles to be deposited on the slides. In
general, average cellular specimens will require a speed of
approximately 1000 rpm with medium acceleration. Very
large cells or fragile cells may require a slower speed, such as
500 to 800 rpm with low acceleration. Specimens consisting
of tiny objects such as bacteria may require much higher
speeds, approaching 2000 rpm with high acceleration.
Time of Cytospin operation is also related to specimen type
and to subsequent preparative steps. For most cytological
preparations, it is desirable to avoid any possibility of
air drying of the specimen. Therefore the time used for
Cytospin operation is kept as short as possible, such as 3 to 4
minutes. For haematology and microbiology specimens that
often are air dried prior to further processing, a longer time
is used, often approaching ten minutes.
An appropriate Cytospin time will ensure fluid absorption.
A78310250ES 5.1ª Edición
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