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Specimen Preparation
Initial Examination
A78310250ES 5.1ª Edición
take into account the subsequent procedures that will be
used. In general, it would be expected that the label might
be subjected to fixation steps and staining procedures.
Obviously, a paper label would be inappropriate. Pencil
identification on frosted-end glass slides is the most
common approach to specimen identification.
The laboratory must also ensure that adequate labelling
is maintained for all containers or devices to which the
specimen is transferred. In use of the Cytospin, this may
include one or more centrifugation steps, conducted in a
standard laboratory centrifuge. Each new container to which
the specimen is transferred must be appropriately labelled.
In addition, the laboratory must ensure containment
of the specimen to eliminate potential hazards to the
laboratory personnel. Since most centrifuges do not provide
aerosol containment during operation, any intermediate
centrifugation steps should be conducted in a biological
safety cabinet. At the conclusion of specimen preparation, all
intermediate containers, pipettes, etc., should be disposed of
in an appropriate biohazard container.
Cytological examination always begins with a macroscopic
examination of the specimen at the time it is submitted to
the laboratory. This is a crucial examination, as it provides
information that will be used to select processing protocol.
The macroscopic examination is most useful in the hands
of an experienced technologist. Prior experience with a
particular specimen is invaluable in recognising whether
a given sample is normal or highly suspect, and whether
the specimen will be adequate for cytological examination.
However, it is usually impossible to determine if a given
sample contains abnormal cells from the macroscopic
examination only. A specimen which should normally be
clear should not be assumed to be abnormal simply because
it is bloody on arrival in the laboratory. Any number of
circumstances may produce a different appearance in a
specimen during the collection process.
Manual de operaciones de la Cytospin 4
Methodology Guidelines
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