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Suggestions for
Optimal Cytospin
Technique
A78310250ES 5.1ª Edición
10 When the 'End of Run' tune has ended, remove the
sealed head and transfer it to a hood before opening.
11 Check the sample chambers to see if all of the specimen
has spun onto the slide. Make sure that any remaining
specimen in the chamber does not flow onto the slide as
it is removed from the assembly. Should this occur, the
cells will not remain spread out on the slide and may
therefore overstain.
1 Use coated slides.
2 Do not make a push smear, even when the cell counts
are high. Large malignant cells are difficult to identify on
push smears because they may aggregate at the feather
edge or stain darkly in the thick portion of the smear.
3 Use a disposable EZ Cytofunnel and do not let unspun
specimen wet the slide.
4 If fibrin strands or other contaminated materials are
present, they may clog the filter card and prevent
absorption of the specimen. Better slides may sometimes
be obtained if an aliquot of the specimen is first diluted
in saline, centrifuged, and then the cells are re-suspended
in saline to the original volume.
5 If synovial fluid is extremely viscous, a small amount of
hyaluronidase may be added to liquefy the sample before
processing.
6 If a fluid is clotted, Cytospin slides may be prepared
from a suspension of the clotted material as well as from
the unclotted fluid to increase the possibility of detecting
malignant cells.
Manual de operaciones de la Cytospin 4
Methodology Guidelines
107
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