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Methodology
Appendix B
Guidelines
General Theory
Introduction
The Cytospin is a special purpose instrument designed to
deposit cells on to glass slides. The instrument produces
monolayer cell deposition in a defined area of the slide,
using centrifugal force. For most cytological specimens the
Cytospin offers significant advantages in specimen retention,
preparation, and standardisation, and ease of specimen
evaluation.
Cytological specimens may also be deposited on to slides
by techniques such as direct smears or by filter techniques.
While useful with some specimens, both direct smears
and filter techniques have significant disadvantages when
compared with Cytospin preparations.
Direct smears consistently produce preparations of varying
thickness from end to end of the smear. In addition, severe
mechanical damage may result to many cells within the
preparation. There is also a likelihood of selective cell
distribution within the smear. Cells of different sizes will be
deposited in different areas of the smear.
Filter preparations, while excellent for cell retention, are
technically demanding and time consuming. In addition,
filter preparations rarely yield slides that can be evaluated
easily. The cells are seldom in the same plane as the focus
within the microscope, and it is extremely difficult to obtain
well stained cells without also staining the filter. For those
filter techniques that dissolve the filter, there is a significant
risk of cell loss, in addition to the difficulty and hazards of
using a volatile and dangerous solvent.
Cytospin preparations effectively circumvent these
difficulties, and consistently produce uniform preparations
A78310250ES 5.1ª Edición
Manual de operaciones de la Cytospin 4
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