Introduction; The Macsquant® Tyto® Cell Sorter - Miltenyi Biotec MACSQuant Tyto Manual Del Usuario

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Introduction

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1.1 The MACSQuant® Tyto® Cell Sorter
The MACSQuant® Tyto® Cell Sorter is an instrument for fluorescence-based cell sorting using a patented
microchip technology with a high-speed mechanical sort valve. The high-frequency fluidic valves embedded in
the closed cartridges have a microsecond range of valve opening and closing cycles. The MACSQuant® Tyto®
Cell Sorter can reach a sort rate up to 30,000 valve actuations per second (results depend on input sample and
Poisson statistics).
Cells pass through the microchip under low positive pressure and no sheath fluid for the gentlest conditions.
This cell isolation method ensures high cell viability and functionality, even with fragile cells. The cartridge has
aseptic ports for easy sample administration and collection. Since the sorting process is not using droplets, no
biohazardous aerosols are formed. These unique features minimize the operator risk. Samples are also protected
from environmental contamination in the closed system of theMACSQuant Tyto Cell Sorter. Disposable
cartridges minimize sample-to-sample carryover and eliminate the need for sample loading and collection
ports. Cells sorted with the MACSQuant Tyto Cell Sorter are ready for use in sensitive downstream applications
such as cell culture and cell expansion.
Figure 1.1: Front view of the MACSQuant Tyto Cell Sorter.
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