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HistoCore
SPECTRA
H&E Staining System
S1
3801654
S2
3801655
CAUTION: The user has 5 minutes to scan the reagents for the staining system packaging label and 5 minutes per reagent
bottle. If reading in a reagent bottle or staining system label fails, the user has 2 additional attempts before the reagent
labels become invalid.
NOTE: To scan a secondary staining system, select a staining reagent labeled S1B/S2B, and follow the directions above
to complete scanning and filling of staining system and reagents.
NOTE: For additional instructions on defining bathlayouts, refer to the SPECTRA ST Instructions for Use.
Refilling Exhausted or Expired Staining Systems
•
To replenish exhausted or expired staining systems, open the "Bathlayout" screen by pressing on the "Bathlayout" tab on
the main menu. Press on any of the staining components. A window will open asking to scan the Leica kit label. Using the
staining system box, hold the label of the staining system box up to the RFID sensor on the front, left side of the instrument.
This will scan the new staining system into the instrument software. After being prompted by a message, scan the first reagent
bottle in the staining system by holding the reagent bottle label in front of the RFID sensor on the front, left of the instrument.
Reagent bottles may be scanned in any order. A new window will open displaying the bathlayout. The reagent that was
scanned in will be outlined in orange on the bathlayout. Remove the reagent vessel from the instrument, open the reagent
bottle, and pour all the reagent into the reagent vessel. Place the reagent vessel back into the respective station. Once this is
completed, confirm filling and placement of the reagent vessel by pressing the respective station on the screen. Repeat this
step until all 5 reagent bottles have been scanned and placed in their respective stations on the instrument. After all reagent
vessels have been scanned in and filled, a window will open instructing to complete a "Fill Level Scan". Press "OK" to complete
the fill level scan.
CAUTION: The user has 5 minutes to scan the reagents for the staining system packaging label and 5 minutes per reagent
bottle. If reading in a reagent bottle or staining system label fails, the user has 2 additional attempts before the reagent
labels become invalid.
Agitation Settings:
Agitation settings are predefined. Agitation will occur at a setting of 4. Refer to the HistoCore SPECTRA ST Instructions for Use.
Immersion Times:
Immersion times and instrument settings for HistoCore SPECTRA ST stainer are detailed in Table 1 below. Please follow instructions
for operating HistoCore SPECTRA ST as detailed in the HistoCore SPECTRA ST Instructions for Use.
CAUTION: Modifications to the validated staining protocol may result in compromised staining quality as well as staining capacity.
CAUTION: All of the reagents in each HistoCore SPECTRA H&E Staining System S1 and S2 must be used together to achieve the
expected performance. Substitution of non-HistoCore SPECTRA H&E reagents or reagents from another HistoCore SPECTRA H&E
Staining System will compromise performance.
CAUTION: Opening the lid of the HistoCore SPECTRA ST stainer during protocol execution may increase time in staining system
reagent and impact the consistency in staining quality.
CAUTION: Reagent vessels should be covered when not in use to reduce evaporation.
CAUTION: If the HistoCore SPECTRA ST is not connected to the HistoCore SPECTRA CV, the slides will be transferred to the unload drawers.
In this occurrence, ensure there is the appropriate reagent in the unload vessel, otherwise quality of staining may be compromised.
CAUTION: Any deviations from these instructions may alter expected results and are neither validated nor supported by Leica Biosystems.
Technical Notes
•
100% alcohol and xylene reagents used for deparaffinization and rehydration should be rotated after every 800 slides.
Reagent rotation is defined as follows:
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Empty most contaminated reagent of a series and refill container.
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Move remaining container(s) up one space in sequence.
•
Place newly filled container into the last position of the series.
•
The 100% alcohol and xylene reagents used for dehydration and clearing before coverslipping also should be rotated after every
800 slides as described above.
•
Two of the four 95% alcohols (steps 5, and 14) should be discarded and replaced with fresh 95% alcohol solution every 800 slides.
•
The two 95% dehydrating alcohols (step 16) should be discarded and replaced with fresh 95% alcohol solution every 400 slides per
reagent vessel.
•
If using the Reagent Management System (RMS), after change and rotation, update the RMS with changes. Instruction for use
of the RMS can be found in the HistoCore SPECTRA ST Instructions for Use.
•
CAUTION: Failure to rotate and change reagents as required may result in inadequate deparaffinization as well as excessive carry-over
and dilution of reagents or solvent.
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