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HistoCore
SPECTRA
H&E Staining System
S1
3801654
S2
3801655
•
NOTE: Reagent vessels (including water rinse vessels) should be inspected at periodic intervals to ensure the integrity and cleanliness
of the vessels. If cleaning solutions are used, rinse vessels with adequate water to remove any residual cleaning solutions prior to use.
Clean the vessels in accordance with the instructions provided in the HistoCore SPECTRA ST Instructions for Use.
NOTE: The HistoCore SPECTRA ST Reagent Management System (RMS) tracks the number of slides that have utilized a specific station.
However, for the ancillary reagents that have been rotated, the RMS will no longer capture the total number of slides that have
utilized that specific ancillary reagent. For more information please refer to the SPECTRA ST Instructions for Use.
Protocol Name: H&E Staining System S1/
Table 1
H&E Staining System S2
Step
Reagent
Default: Include
Oven Step
1
Xylene Dewax 1
2
Xylene Dewax 2
3
100% Alcohol Dewax 1
4
100% Alcohol Dewax 2
5
95% Alcohol Dewax 1
6
Tap Water
SPECTRA Hemalast S1/
7
SPECTRA Hemalast S2
SPECTRA Hematoxylin S1/
8
SPECTRA Hematoxylin S2
9
Tap Water
SPECTRA Differentiator S1/
10
SPECTRA Differentiator S2
11
Tap Water
SPECTRA Bluing Agent S1/
12
SPECTRA Bluing Agent S2
13
Tap Water
14
95% Alcohol Neutralizing H
SPECTRA Eosin S1/
15
SPECTRA Eosin S2
16*
95% Alcohol Dehyd 1
17
100% Alcohol Dhyd 1 H
18
100% Alcohol Dhyd 2 H
19
Xylene Dehyd 1
20
Xylene Dehyd 2
21
* Indicates a manually duplicated step.
Abbreviation
Oven
Oven
XylDwx 1
XylDwx 2
100Dwx 1
100Dwx 2
95Dwx 1
Tap Water
L HLst S1/
L HLst S2
L Hmtx S1/
L Hmtx S2
Tap Water
L Diff S1/
L Diff S2
Tap Water
L Blue S1/
L Blue S2
Tap Water
95Neutr H
L Eos S1/
L Eos S2
95Dhy 1
100Dhy 1H
100Dhy 2H
XylDhy 1
XylDhy 2
Exit
NA
Abbreviation: HE1/HE2
Time (mm:ss)
Tolerance
Process Class
15:00
50%
2:00
100%
2:00
100%
2:00
100%
2:00
100%
2:00
100%
2:00
100%
2:00
100%
Refer to
Hematoxylin
0%
Dial Settings
2:00
100%
S1: 1:30
0%
Differentiating
S2: 1:00
2:00
100%
2:00
100%
2:00
100%
2:00
100%
Refer to Eosin
0%
Dial Settings
2:00
0%
Dehydrating
2:00
0%
Dehydrating
2:00
0%
Dehydrating
2:00
100%
Dehydrating
2:00
100%
Dehydrating
NA
NA
Page 7 of 193
Agitation Speed: 4
Heated Vessel
NA
NA
Dewaxing
No
Dewaxing
No
Dewaxing
No
Dewaxing
No
Dewaxing
No
NA
No
Neutralizing
No
Staining
No
NA
No
No
NA
No
Staining
No
NA
No
Neutralizing
No
Staining
No
No
No
No
No
No
NA
NA
Oven Temperature: 70°C
Days Max
Slide Max
NA
NA
5
800
5
800
5
800
5
800
5
800
NA
NA
7
1600
7
1600
NA
NA
7
1600
NA
NA
7
1600
NA
NA
5
800
7
1600
5
400
5
800
5
800
5
800
5
800
NA
NA
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