helena BioSciences SAS-1 Pentavalent Screen Instrucciones De Uso página 6

b) SAS-4 (Auto-Stainer)
Step
Wash
Wash
Stain
Destain
Destain
Dry
I I N N T T E E R R P P R R E E T T A A T T I I O O N N O O F F R R E E S S U U L L T T S S
The majority of monoclonal proteins migrate in the cathodic, gamma region of the protein pattern, but
due to their abnormal nature, they may migrate anywhere within the globulin region on protein
electrophoresis. The abnormal protein should now identified with a full IFE.
When low concentrations of abnormal protein are present, the abnormal band may appear as a band
within the normal polyclonal immunoglobulin. A band can also be seen within a polyclonal background
when there is a large polyclonal immunoglobulin presence also.
Q Q U U A A L L I I T T Y Y C C O O N N T T R R O O L L
Kemtrol Abnormal (Cat. No. 7025) will give a single monclonal band, within polyclonal background.
L L I I M M I I T T A A T T I I O O N N S S
1 1 . . A A n n t t i i g g e e n n E E x x c c e e s s s s . .
Antigen excess will occur if there is not a slight antibody excess or antigen/antibody equivalence at
the site of precipitation. Antigen excess in IFE is usually due to an excess of the immunoglobulin
in the patient sample. Antigen excess is characterised by prozoning (unstained areas in the centre
of the immunofixed protein band, with staining around the edges). A higher dilution of the sample
should be used in this event to optimise the immunoglobulin concentration.
P P E E R R F F O O R R M M A A N N C C E E C C H H A A R R A A C C T T E E R R I I S S T T I I C C S S
a a ) ) S S e e n n s s i i t t i i v v i i t t y y
Using the urine method (10 applications), 10mg/L was determined as the lowest concentration of
protein which was evident as a discrete band on the completed gel.
b b ) ) L L i i n n e e a a r r i i t t y y
Linearity is a function of densitometer specification as well as gel performance. It is recommended
that each customer determine the linearity of the method based upon the densitometer in use in
the laboratory.
Time (mm:ss)
00:03
10:00
04:00
02:00
02:00
12:00
SAS-1 PENTAVALENT SCREEN
Temp (° ° C C )
63
5
Other
Recirculate ON
Recirculate ON
Recirculate ON
Recirculate ON
Recirculate ON
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