High-Range Heparinase (Hr-Htc); General Purpose Cartridge (Gpc) - Medtronic ACT Plus Manual Del Usuario

ACT_CH.fm 6/23/04 1:23 pm
UC200304151c ML
5.5" x 7.75" (139.7 mm x 196.85 mm)
Channel 1 clotting time
Channel 2 clotting time
Mean clotting time
Difference
12%
The difference of 10 seconds is less than 12%, 25 seconds. These results are
acceptable. The instrument's operable range is 25 to 999 seconds.

High-Range Heparinase (HR-HTC)

The HR-HTC cartridge is a modification of the High-Range Activated Clotting Time
Cartridge used to identify the presence of heparin in a fresh whole blood sample.
One of the cartridge channels contains a purified bacterial heparinase. The
heparinase rapidly and specifically destroys up to 6 U/mL of heparin present in the
sample.
1. Shake or tap the HR-HTC cartridge to resuspend the reagent before use, and
prewarm it for 3 to 5 minutes (up to a maximum of 2 hours).
2. Fill each chamber with 0.4 mL of sample (the sample level should be between
the upper and lower fill lines in each chamber).
Note: To ensure that no heparinase is inadvertently transferred between
channels, fill the channel labeled with "HR" first, and the channel that is
stamped with the purple "HR-HTC" second.
3. Initiate the test immediately by rotating the actuator heat block to the closed
position.
Notes:
■ The HR-HTC displays two results: one for the sample with heparinase and
one for the sample without heparinase.
The difference between the two channels indicates the effect of heparin on
■
the clotting time. The baseline clotting time may or may not be at or near the
normal range. If the baseline is not within the normal range in the HR-HTC
channel, some condition other than the presence of heparin is responsible for
extending the baseline clotting time.

General Purpose Cartridge (GPC)

The General Purpose Cartridge is used to perform Prothrombin Time (PT) or
Activated Partial Thromboplastin Time (APTT) tests on citrated whole blood or
plasma samples. Each channel of a GPC contains 100 µL of 0.02 M calcium
chloride. User-defined reagents specific for PT and APTT testing are added to the
cartridge channels before the patient sample is added.
36 English
86702-001 Rev 1.0
Sample Calculation:
210 seconds
200 seconds
205 seconds
10 seconds
25 seconds
ACT Plus™ Operator's Manual
Medtronic Confidential
CS010