Abaxis VETSCAN VS2 Manual Del Usuario página 11

1.3.3 Step 3: Add Sample to the Rotor
Sample Delivery Volume
Follow these guidelines to make sure you add the correct sample volume to the reagent rotor.
When a sufficient quantity of sample is added, it will

form a line between the arrows on the rotor surface.
If an air bubble is introduced, add enough sample to

fill the overflow area by 1/3 to 1/2, as shown at right.
Make sure any excess sample does not travel beyond

the overflow area.
Using a Fixed 100 µL Volume Pipette
1. Firmly attach a new tip to the end of the pipette. Do not
touch the tip, or a false elevation of amylase can result.
2. With your index finger or thumb, push the pipette plunger to the stop
position, and hold it there for sample pickup.
3. Immerse the tip 2–3 mm below the surface of the sample, as shown at
right.
4. Slowly release the plunger to draw the sample. Pause, then remove the
pipette from the sample tube.
5. Make sure there are no air bubbles or gaps in the pipette tip.
6. Place the pipette tip into the rotor's sample port, so that the tip just
touches the sample port, as shown at right.
7. Gently and slowly depress the pipette plunger to allow sample to
slowly fill the sample chamber. See
page 1-7 for the appropriate sample delivery volume.
Note: Keep the pipette plunger pressed down until you remove the tip from the sample port.
"Sample Delivery Volume" on
Quick Reference Guide
90 μL
> 90 μL
< 120 μL
<90 μL
> 120 μL
1-7