direction in the drawer. The analysis begins when
the drawer closes.
5.
Input patient and operator identification numbers as
directed by the messages on the analyzer display.
The analyzer processes the sample with no further
input from the operator. The entire analysis takes
< 15 minutes and is complete when an
message appears on the display.
complete
6.
The results are stored in the analyzer. Print the
results, transmit to a computer, or touch
bypass this step. To transmit, see
External Computer" on page
7.
The drawer automatically opens. Remove the rotor
from the drawer and follow standard hazardous
waste disposal procedures when disposing the rotor.
The rotor may be placed back into the pouch before
disposal. Touch
Close
to be run immediately.
C.10 Timing Considerations
• Analyze whole blood samples collected by venipunc-
ture within 60 minutes of collection.
• Run the reagent rotor within 10 minutes of applying
sample or control to the rotor.
• Dispense samples collected in micropipettes into the
rotor immediately after collection.
C.11 Quality Control
Although elaborate quality controls are integrated into
the VetScan VS2 Chemistry Analyzer and reagent pro-
file rotors, the analyzer's performance can also be veri-
fied by running external controls. This can be
accomplished by testing an aliquoted sample with
known values, or by using a serum-based, commercially
available control. See
"Running Controls" on page
C.12 Calibration
The VetScan VS2 Chemistry Analyzer is calibrated by
the manufacturer before shipment. The bar code printed
on the bar code ring provides the analyzer with rotor-
specific calibration data. See
Quality Control" on page
C.13 Results
The VetScan VS2 Chemistry Analyzer automatically
calculates and prints the analyte concentrations in the
sample. Details of the endpoint and rate reaction calcula-
tions are found in
"Principles of Procedure and Opera-
tion" on page
9-1.
C-6
Analysis
Open
"Connecting to an
11-1.
if another reagent rotor is not
"Calibration and
7-1.
Methods Performance
The VetScan analyzer calculates the globulin concentra-
tion using the total protein and albumin concentrations
determined by the chemical analyses. If the total protein
or albumin results are out of range or suppressed, the
globulin concentration cannot be reliably calculated and
is not reported.
C.14 Messages Printed in Results
• Results outside the reference range are indicated in the
results by an asterisk ( * ) next to the analyte concen-
to
tration.
• Results outside the dynamic range are indicated in the
results by a greater-than symbol ( > ) next to the high-
est value of the dynamic range, or a less-than symbol
( < ) next to the lowest value of the dynamic range.
(For example, concentrations outside an analyte
dynamic range are printed as > 700 or < 10, respec-
tively.)
• "HEM", "LIP", or "ICT" is printed in place of the ana-
lyte concentration if hemolysis (HEM), lipemia (LIP),
or icterus (ICT) adversely affects the results for that
analyte. LIP is also printed if both lipemia and icterus
affect a result. HEM is also printed if hemolysis and
icterus, hemolysis and lipemia, or hemolysis, lipemia,
and icterus affect a particular analyte. Examine the
sample indices to determine if more than one interfer-
ent is affecting a particular result.
• The sample indices are printed in the results. The indi-
ces indicate the degree of hemolysis, icterus, and
lipemia in the sample, measured on a scale of 0
(clear), 1+ (slight), 2+ (moderate), and 3+ (gross).
C.15 Limitations of Procedure
• Lithium heparin is the only anticoagulant recom-
mended for use with the VetScan VS2 Chemistry Ana-
7-3.
lyzer.
• If a result for a particular test exceeds the assay range,
the sample may be able to be diluted (see
shooting" on page
out of range, have it analyzed by another approved test
method or sent to a referral laboratory. Do not dilute
the sample and run it a third time.
• If sample or reagent rotor errors are indicated on the
analyzer display or printed in the results, consult
"Troubleshooting" on page 8-1
the error message.
• Samples with high HCT do not reprint as hemolyzed.
Typically report cancellation code.
"Trouble-
8-1). If the sample again appears
for an explanation of