Section 9 Principles Of Procedure And Operation; Principles Of Procedure - Abaxis VETSCAN VS2 Manual Del Usuario

Principles of
Section 9
Procedure and
Operation
9.1

Principles of Procedure

The operator introduces a heparinized whole blood sample (or
heparinized plasma, serum, or control) into the reagent rotor. The
reagent rotor contains a diluent, and test-specific reagent beads.
The operator then places the rotor in the VetScan VS2 Chemistry
Analyzer and enters the appropriate identification numbers. The
VS2 Analyzer automatically performs the remainder of the test-
ing protocol.
The reagent rotor spins and whole blood is separated into plasma
and blood cells. During this time, the rotor is heated to 37 ºC
(98.6 ºF). Precisely measured quantities of plasma and diluent
enter the mixing chamber and are combined. Centrifugal and
capillary forces distribute the diluted plasma to cuvettes on the
perimeter of the rotor. Reagent beads in the cuvettes are dis-
solved by the diluted plasma. This solution is thoroughly mixed,
and the resulting chemical reactions are monitored photometri-
cally by the analyzer. Optical signals generated by the chemical
reactions are used to calculate analyte concentrations. Calibra-
tion data specific for the chemistries in each rotor are provided to
the analyzer by the bar code printed on the bar code ring.
After analysis is complete, the results print automatically, and
include analyte concentrations and reference ranges for each test
in the panel, as well as sample indices. Results are also stored in
the analyzer's memory and can be transmitted to external com-
puters. The used reagent rotor is removed and discarded. The
VS2 is ready to analyze another sample.
Principles of Procedure and Operation
9-1