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11. Incubation or mixing times or temperatures other than those specified may give erroneous results.
12.Erroneous results may occur if the flow cytometer is not properly aligned or standardized for fluorescence or
if the cell populations are improperly gated.
13.Results determined using flow cytometers, lysing systems, or antibodies that are different from those used to
determine the expected results may not fall within the expected range.
14.Use good laboratory practices when handling this reagent.
GHS HAZARD CLASSIFICATION
Not classified as hazardous
STORAGE, STABILITY, AND DISPOSAL
•
Reagent stability: this reagent is stable up to the expiration date stated on the vial label when stored unopened
at 2 – 8°C. Do not use after expiration date. Open vial stability is 30 days.
Opened vials must be capped tightly and stored at 2 – 8°C after use. Do not freeze.
•
Bring Stem-Trol Control Cells to room temperature (18 – 25°C) prior to use.
IMPORTANT:
Risk of erroneous results if leakage of the Stem-Trol Control Cells occurs. Once opened,
Stem-Trol Control Cells must be stored in an upright position to prevent the possibility of
leakage. Reagents should not be used if signs of leakage are observed.
Evidence of Deterioration
Inability to obtain expected results or a shift in light scatter or fluorescence properties may indicate product
deterioration. Instrument standardization, sample preparation technique, and antibody performance should also
be investigated.
Any change in the physical appearance of Stem-Trol Control Cells (normal appearance is a colorless liquid) or
any major variation (>15% change in absolute count) in values obtained from replicated tests by flow cytometry
analysis, may indicate deterioration and the reagent should not be used further.
Reagent Preparation
Stem-Trol Control Cells is a ready-to-use cell suspension. Proper mixing (vortex for 10 – 12 seconds) is required
before each first pipetting from the vial. Avoid excessive mixing to minimize formation of air bubbles. Do not pipet
air bubbles.
MATERIALS REQUIRED, BUT NOT PROVIDED
•
Deionized water.
•
Stem-Kit Reagents (Ref. IM3630), including 7-AAD Viability Dye.
•
As an alternative to Stem-Count Fluorospheres: Flow-Count Fluorospheres (Ref. 7547053)
•
Ice water bath.
•
Plastic test tubes (12 x 75 mm).
•
Calibrated positive displacement pipet (20 µL, 100 µL, 2 mL) and tips or calibrated repeater pipet (20 µL, 100
µL, 2 mL) and tips.
•
Calibrated standard pipets (20 µL, 100 µL, 2 mL) and tips.
•
Vortex mixer.
•
Timer.
•
Flow cytometer.
•
stemCXP System Software ONLY for automated analysis of Stem-Trol Control Cells on FC 500 flow
cytometers equipped with CXP Software.
PROCEDURE
For automatic analysis, refer to the stemCXP System Guide provided with the stemCXP System Software for
complete instructions.
For manual analysis, refer to the Stem-Kit Reagents package insert and the following procedure.
B60231–AE
Safety Data Sheet is available at
beckman.com/techdocs
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